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AF4/FMR2 Family, Member 4 (AFF4)

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MCEF; AF5Q31; Major Cdk9-Interacting Elongation Factor; ALL1 Fused Gene From 5q31

AF4/FMR2 Family, Member 4 (AFF4)
CCC J719
HGNC 17869
MGI 2136171
PubMed 20159561
RGD 1311694
UniProt Q9UHB7
MCEF or Major Cdk9-interacting elongation factor is a transcription factor related to Af4. It is the fourth member of the Af4 family (AFF) of trancription factors, involved in numerous pathologies, including Acute Lymphoblastic Leukemia (ALL), abnormal CNS development, breast cancer and azoospermia.
Because it apparently interacts with the species-specific human co-factor (P-TEFb) for HIV-1 transcription, and because it can repress HIV-1 replication, MCEF (also known as AFF4 or AF5q31) may have future therapeutic uses.MCEF was originally cloned and named by Mario Clemente Estable of Ryerson University, while he was a post-doctoral fellow in the laboratory of Robert G. Roeder, at the Rockefeller University.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant AF4/FMR2 Family, Member 4 (AFF4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to AF4/FMR2 Family, Member 4 (AFF4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to AF4/FMR2 Family, Member 4 (AFF4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for AF4/FMR2 Family, Member 4 (AFF4) CLIA Kit Customized Service Offer
n/a ELISA Kit for AF4/FMR2 Family, Member 4 (AFF4) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant AF4/FMR2 Family, Member 4 (AFF4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to AF4/FMR2 Family, Member 4 (AFF4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to AF4/FMR2 Family, Member 4 (AFF4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for AF4/FMR2 Family, Member 4 (AFF4) CLIA Kit Customized Service Offer
n/a ELISA Kit for AF4/FMR2 Family, Member 4 (AFF4) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant AF4/FMR2 Family, Member 4 (AFF4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to AF4/FMR2 Family, Member 4 (AFF4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to AF4/FMR2 Family, Member 4 (AFF4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for AF4/FMR2 Family, Member 4 (AFF4) CLIA Kit Customized Service Offer
n/a ELISA Kit for AF4/FMR2 Family, Member 4 (AFF4) ELISA Kit Customized Service Offer
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  2. "MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein."J. Biomed. Sci. 9:234-245(2002) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and comparative analysis of human chromosome 5." Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
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  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  8. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  9. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  11. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  15. "HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription."Mol. Cell 38:428-438(2010) [PubMed] [Europe PMC] [Abstract]
  16. "AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia."Mol. Cell 37:429-437(2010) [PubMed] [Europe PMC] [Abstract]
  17. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  18. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  19. "The little elongation complex regulates small nuclear RNA transcription."Mol. Cell 44:954-965(2011) [PubMed] [Europe PMC] [Abstract]
  20. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  21. "The ubiquitin ligase Siah1 controls ELL2 stability and formation of super elongation complexes to modulate gene transcription."Mol. Cell 46:325-334(2012) [PubMed] [Europe PMC] [Abstract]
  22. "The super elongation complex (SEC) family in transcriptional control."Nat. Rev. Mol. Cell Biol. 13:543-547(2012) [PubMed] [Europe PMC] [Abstract]
  23. "HIV-1 Tat recruits transcription elongation factors dispersed along a flexible AFF4 scaffold."Proc. Natl. Acad. Sci. U.S.A. 110:E123-E131(2013) [PubMed] [Europe PMC] [Abstract]
  24. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  25. "Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma." Nature 469:539-542(2011) [PubMed] [Europe PMC] [Abstract]