Peflin (PEF1)
[Edit]PEF1A; ABP32; Penta-EF-Hand Domain Containing 1; PEF protein with a long N-terminal hydrophobic domain
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PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF hand (PEF) protein family. The deduced 284-amino acid protein has a calculated molecular mass of 30.3 kD. PEF1 contains an N-terminal hydrophobic domain, 5 C-terminal EF-hand motifs, and several potential phosphorylation sites. It shares about 30% amino acid identity with ALG2.
Peflin dissociated from ALG2 in the presence of Ca(2+), and the N-terminal hydrophobic domain of peflin was not essential for heterodimerization. Peflin and ALG2 colocalized in the cytoplasm, but ALG2 was also detected in nuclei, as revealed by immunofluorescence staining and subcellular fractionation. Peflin was recovered in the cytosolic fraction in the absence of Ca(2+) and in the membrane/cytoskeletal fraction in the presence of Ca(2+).
Organism species: Homo sapiens (Human)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | n/a | Recombinant Peflin (PEF1) | Recombinant Protein Customized Service Offer |
| Antibodies | n/a | Monoclonal Antibody to Peflin (PEF1) | Monoclonal Antibody Customized Service Offer |
| n/a | Polyclonal Antibody to Peflin (PEF1) | Polyclonal Antibody Customized Service Offer | |
| Assay Kits | n/a | CLIA Kit for Peflin (PEF1) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for Peflin (PEF1) | ELISA Kit Customized Service Offer |
Organism species: Mus musculus (Mouse)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | n/a | Recombinant Peflin (PEF1) | Recombinant Protein Customized Service Offer |
| Antibodies | n/a | Monoclonal Antibody to Peflin (PEF1) | Monoclonal Antibody Customized Service Offer |
| n/a | Polyclonal Antibody to Peflin (PEF1) | Polyclonal Antibody Customized Service Offer | |
| Assay Kits | n/a | CLIA Kit for Peflin (PEF1) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for Peflin (PEF1) | ELISA Kit Customized Service Offer |
Organism species: Rattus norvegicus (Rat)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | n/a | Recombinant Peflin (PEF1) | Recombinant Protein Customized Service Offer |
| Antibodies | n/a | Monoclonal Antibody to Peflin (PEF1) | Monoclonal Antibody Customized Service Offer |
| n/a | Polyclonal Antibody to Peflin (PEF1) | Polyclonal Antibody Customized Service Offer | |
| Assay Kits | n/a | CLIA Kit for Peflin (PEF1) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for Peflin (PEF1) | ELISA Kit Customized Service Offer |
- "Peflin, a novel member of the five-EF-hand-protein family, is similar to the apoptosis-linked gene 2 (ALG-2) protein but possesses nonapeptide repeats in the N-terminal hydrophobic region."Biochem. Biophys. Res. Commun. 263:68-75(1999) [PubMed] [Europe PMC] [Abstract]
- "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
- "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "Peflin and ALG-2, members of the penta-EF-hand protein family, form a heterodimer that dissociates in a Ca2+-dependent manner."J. Biol. Chem. 276:14053-14058(2001) [PubMed] [Europe PMC] [Abstract]
- "Both ALG-2 and peflin, penta-EF-hand (PEF) proteins, are stabilized by dimerization through their fifth EF-hand regions."Arch. Biochem. Biophys. 399:12-18(2002) [PubMed] [Europe PMC] [Abstract]
- "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]