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DNA Repair Protein RAD50 (RAD50)

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DNA Repair Protein RAD50 (RAD50)
CCC K111
HGNC 9816
MGI 109292
PubMed 12152085
RGD 621542
UniProt Q92878
Wiki RAD50

The S. cerevisiae Rad50 gene encodes a protein that is essential for double-stranded DNA break repair by nonhomologous DNA end joining and chromosomal integration.

The human RAD50 gene spans 100 to 130 kb. Northern blot analysis revealed that the RAD50 gene was expressed as a 5.5-kb mRNA predominantly in testis. A faint 7-kb transcript, which the authors considered to be an mRNA with an alternatively processed 3-prime end, was also detected. Yeast Rad50 and the predicted 1,312-amino acid human RAD50 protein share more than 50% identity in their N- and C-termini. The central heptad repeat domains of the proteins have relatively divergent primary sequences but are predicted to adopt very similar coiled-coil structures.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPK111Hu01 Recombinant DNA Repair Protein RAD50 (RAD50) Positive Control; Immunogen; SDS-PAGE; WB.
RPK111Hu02 Recombinant DNA Repair Protein RAD50 (RAD50) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAK111Hu01 Polyclonal Antibody to DNA Repair Protein RAD50 (RAD50) WB; IHC; ICC; IP.
PAK111Hu02 Polyclonal Antibody to DNA Repair Protein RAD50 (RAD50) WB; IHC; ICC; IP.
LAK111Hu71 Biotin-Linked Polyclonal Antibody to DNA Repair Protein RAD50 (RAD50) WB; IHC; ICC.
Assay Kits SEK111Hu ELISA Kit for DNA Repair Protein RAD50 (RAD50) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPK111Mu01 Recombinant DNA Repair Protein RAD50 (RAD50) Positive Control; Immunogen; SDS-PAGE; WB.
RPK111Mu02 Recombinant DNA Repair Protein RAD50 (RAD50) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAK111Mu01 Polyclonal Antibody to DNA Repair Protein RAD50 (RAD50) WB; IHC; ICC; IP.
PAK111Mu02 Polyclonal Antibody to DNA Repair Protein RAD50 (RAD50) WB; IHC; ICC; IP.
Assay Kits SEK111Mu ELISA Kit for DNA Repair Protein RAD50 (RAD50) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPK111Ra01 Recombinant DNA Repair Protein RAD50 (RAD50) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to DNA Repair Protein RAD50 (RAD50) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to DNA Repair Protein RAD50 (RAD50) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for DNA Repair Protein RAD50 (RAD50) CLIA Kit Customized Service Offer
n/a ELISA Kit for DNA Repair Protein RAD50 (RAD50) ELISA Kit Customized Service Offer
  1. "Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair."Mol. Cell. Biol. 16:4832-4841(1996) [PubMed] [Europe PMC] [Abstract]
  2. "Molecular cloning and characterization of splice variants of human RAD50 gene."Gene 235:59-67(1999) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and comparative analysis of human chromosome 5." Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response."Cell 93:477-486(1998) [PubMed] [Europe PMC] [Abstract]
  6. "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95."J. Biol. Chem. 273:21447-21450(1998) [PubMed] [Europe PMC] [Abstract]
  7. "The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks."Mol. Cell 1:969-979(1998) [PubMed] [Europe PMC] [Abstract]
  8. "Association of BRCA1 with the hRad50-hMre11-p95 complex and the DNA damage response."Science 285:747-750(1999) [PubMed] [Europe PMC] [Abstract]
  9. "BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures."Genes Dev. 14:927-939(2000) [PubMed] [Europe PMC] [Abstract]
  10. "Functional link between ataxia-telangiectasia and Nijmegen breakage syndrome gene products."Nature 405:473-477(2000) [PubMed] [Europe PMC] [Abstract]
  11. "Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres."Nat. Genet. 25:347-352(2000) [PubMed] [Europe PMC] [Abstract]
  12. "RINT-1, a novel Rad50-interacting protein, participates in radiation-induced G2/M checkpoint control."J. Biol. Chem. 276:6105-6111(2001) [PubMed] [Europe PMC] [Abstract]
  13. "Human Rad50/Mre11 is a flexible complex that can tether DNA ends."Mol. Cell 8:1129-1135(2001) [PubMed] [Europe PMC] [Abstract]
  14. "Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex."Nature 418:348-352(2002) [PubMed] [Europe PMC] [Abstract]
  15. "DNA end-binding specificity of human Rad50/Mre11 is influenced by ATP."Nucleic Acids Res. 30:4425-4431(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Artemis is a phosphorylation target of ATM and ATR and is involved in the G2/M DNA damage checkpoint response."Mol. Cell. Biol. 24:9207-9220(2004) [PubMed] [Europe PMC] [Abstract]
  17. "Direct activation of the ATM protein kinase by the Mre11/Rad50/Nbs1 complex."Science 304:93-96(2004) [PubMed] [Europe PMC] [Abstract]
  18. "Ataxia-telangiectasia-mutated dependent phosphorylation of Artemis in response to DNA damage."Cancer Sci. 96:134-141(2005) [PubMed] [Europe PMC] [Abstract]
  19. "ATM-dependent phosphorylation of ATF2 is required for the DNA damage response."Mol. Cell 18:577-587(2005) [PubMed] [Europe PMC] [Abstract]
  20. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  21. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder."Am. J. Hum. Genet. 84:605-616(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  24. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  25. "Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex."J. Virol. 84:12504-12514(2010) [PubMed] [Europe PMC] [Abstract]
  26. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  27. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  28. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  29. "Mutation screening of Mre11 complex genes: indication of RAD50 involvement in breast and ovarian cancer susceptibility."J. Med. Genet. 40:E131-E131(2003) [PubMed] [Europe PMC] [Abstract]