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RAD1 Homolog (RAD1)

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HRAD1; REC1; Cell Cycle Checkpoint Protein Hrad1; Rad1-Like DNA Damage Checkpoint; DNA Repair Exonuclease REC1

RAD1 Homolog (RAD1)
CCC K281
HGNC 9806
MGI 1316678
PubMed 23299942
RGD 1306496
UniProt O60671
Wiki RAD1
The cDNAs encode the 282-amino acid RAD1 isoform, which is 90% and 27% identical to mouse Rad1 and S. pombe rad1+, respectively.In the fission yeast S. pombe, the rad1+ gene product is required for DNA repair and replication. The longer cDNA, called Hrad1A, encodes a 282-amino acid polypeptide, while Hrad1B encodes a 163-amino acid polypeptide. Northern blot analysis revealed that human RAD1 is expressed as mRNAs of 5, 3, and 1.3 kb in a variety of human tissues, with higher levels present in some cancer cell lines. Northern blot analysis of cells subjected to ultraviolet radiation demonstrated that human RAD1 expression is not induced in response to DNA damage. Purified RAD1 exhibited terminal exonuclease activity on double-stranded DNA, with a preference for 3-prime ends.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RAD1 Homolog (RAD1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RAD1 Homolog (RAD1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RAD1 Homolog (RAD1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RAD1 Homolog (RAD1) CLIA Kit Customized Service Offer
n/a ELISA Kit for RAD1 Homolog (RAD1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RAD1 Homolog (RAD1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RAD1 Homolog (RAD1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RAD1 Homolog (RAD1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RAD1 Homolog (RAD1) CLIA Kit Customized Service Offer
n/a ELISA Kit for RAD1 Homolog (RAD1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RAD1 Homolog (RAD1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RAD1 Homolog (RAD1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RAD1 Homolog (RAD1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RAD1 Homolog (RAD1) CLIA Kit Customized Service Offer
n/a ELISA Kit for RAD1 Homolog (RAD1) ELISA Kit Customized Service Offer
  1. "Human and mouse homologs of Schizosaccharomyces pombe rad1(+) and Saccharomyces cerevisiae RAD17: linkage to checkpoint control and mammalian meiosis."Genes Dev. 12:2560-2573(1998) [PubMed] [Europe PMC] [Abstract]
  2. "A human and mouse homolog of the Schizosaccharomyces pombe rad1+ cell cycle checkpoint control gene."Genomics 54:331-337(1998) [PubMed] [Europe PMC] [Abstract]
  3. "RAD1, a human structural homolog of the Schizosaccharomyces pombe RAD1 cell cycle checkpoint gene."Genomics 54:344-347(1998) [PubMed] [Europe PMC] [Abstract]
  4. "cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster."Genomics 54:424-436(1998) [PubMed] [Europe PMC] [Abstract]
  5. "A human homologue of the Schizosaccharomyces pombe rad1+ checkpoint gene encodes an exonuclease."J. Biol. Chem. 273:18332-18339(1998) [PubMed] [Europe PMC] [Abstract]
  6. "HRAD1 and MRad1 encode mammalian homologues of the fission yeast rad1+ cell cycle checkpoint control gene."Nucleic Acids Res. 26:3971-3976(1998) [PubMed] [Europe PMC] [Abstract]
  7. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  9. "The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1."Mol. Biol. Cell 10:1985-1995(1999) [PubMed] [Europe PMC] [Abstract]
  10. "Physical interaction among human checkpoint control proteins HUS1p, RAD1p, and RAD9p, and implications for the regulation of cell cycle progression."Genomics 65:24-33(2000) [PubMed] [Europe PMC] [Abstract]
  11. "HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins."J. Biol. Chem. 275:27909-27916(2000) [PubMed] [Europe PMC] [Abstract]
  12. "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9."J. Biol. Chem. 275:29767-29771(2000) [PubMed] [Europe PMC] [Abstract]
  13. "The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9."Biochem. Biophys. Res. Commun. 287:932-940(2001) [PubMed] [Europe PMC] [Abstract]
  14. "Identification and characterization of a paralog of human cell cycle checkpoint gene HUS1."Genomics 79:487-492(2002) [PubMed] [Europe PMC] [Abstract]
  15. "Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue."Cancer Res. 63:5291-5298(2003) [PubMed] [Europe PMC] [Abstract]
  16. "Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene."Genomics 82:644-651(2003) [PubMed] [Europe PMC] [Abstract]
  17. "Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro."Proc. Natl. Acad. Sci. U.S.A. 100:1633-1638(2003) [PubMed] [Europe PMC] [Abstract]
  18. "The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair."Nucleic Acids Res. 32:3316-3324(2004) [PubMed] [Europe PMC] [Abstract]
  19. "The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1."Proc. Natl. Acad. Sci. U.S.A. 101:16762-16767(2004) [PubMed] [Europe PMC] [Abstract]
  20. "The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery."Biochem. J. 389:13-17(2005) [PubMed] [Europe PMC] [Abstract]
  21. "The two DNA clamps Rad9/Rad1/Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity."J. Mol. Biol. 353:980-989(2005) [PubMed] [Europe PMC] [Abstract]
  22. "Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells."Oncogene 24:4728-4735(2005) [PubMed] [Europe PMC] [Abstract]
  23. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  24. "A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling."Science 332:1313-1317(2011) [PubMed] [Europe PMC] [Abstract]
  25. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]