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RAD9 Homolog (RAD9)

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RAD9A; DNA repair exonuclease rad9 homolog A; Cell cycle checkpoint control protein RAD9A

RAD9 Homolog (RAD9)
CCC K283
HGNC 9827
MGI 1328356
PubMed 23468651
UniProt Q99638
Wiki RAD9A
RAD9 is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. In vivo, the human RAD9 protein was phosphorylated in response to DNA damage, suggesting that it participates in a DNA damage-inducible signaling pathway. Immunoprecipitation studies demonstrated that the fully modified form of RAD9 interacts selectively with RAD1 and HUS1 in a stable complex.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RAD9 Homolog (RAD9) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RAD9 Homolog (RAD9) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RAD9 Homolog (RAD9) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RAD9 Homolog (RAD9) CLIA Kit Customized Service Offer
n/a ELISA Kit for RAD9 Homolog (RAD9) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant RAD9 Homolog (RAD9) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to RAD9 Homolog (RAD9) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to RAD9 Homolog (RAD9) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for RAD9 Homolog (RAD9) CLIA Kit Customized Service Offer
n/a ELISA Kit for RAD9 Homolog (RAD9) ELISA Kit Customized Service Offer
  1. "A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene."Proc. Natl. Acad. Sci. U.S.A. 93:13890-13895(1996) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling."J. Biol. Chem. 278:24428-24437(2003) [PubMed] [Europe PMC] [Abstract]
  5. "The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1."Mol. Biol. Cell 10:1985-1995(1999) [PubMed] [Europe PMC] [Abstract]
  6. "Physical interaction among human checkpoint control proteins HUS1p, RAD1p, and RAD9p, and implications for the regulation of cell cycle progression."Genomics 65:24-33(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Human DNA damage checkpoint protein hRAD9 is a 3' to 5' exonuclease."J. Biol. Chem. 275:7451-7454(2000) [PubMed] [Europe PMC] [Abstract]
  8. "HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins."J. Biol. Chem. 275:27909-27916(2000) [PubMed] [Europe PMC] [Abstract]
  9. "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9."J. Biol. Chem. 275:29767-29771(2000) [PubMed] [Europe PMC] [Abstract]
  10. "The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9."Biochem. Biophys. Res. Commun. 287:932-940(2001) [PubMed] [Europe PMC] [Abstract]
  11. "c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage."Mol. Cell. Biol. 22:3292-3300(2002) [PubMed] [Europe PMC] [Abstract]
  12. "Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue."Cancer Res. 63:5291-5298(2003) [PubMed] [Europe PMC] [Abstract]
  13. "Protein kinase Cdelta is responsible for constitutive and DNA damage-induced phosphorylation of Rad9."EMBO J. 22:1431-1441(2003) [PubMed] [Europe PMC] [Abstract]
  14. "Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro."Proc. Natl. Acad. Sci. U.S.A. 100:1633-1638(2003) [PubMed] [Europe PMC] [Abstract]
  15. "The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair."Nucleic Acids Res. 32:3316-3324(2004) [PubMed] [Europe PMC] [Abstract]
  16. "The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1."Proc. Natl. Acad. Sci. U.S.A. 101:16762-16767(2004) [PubMed] [Europe PMC] [Abstract]
  17. "The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery."Biochem. J. 389:13-17(2005) [PubMed] [Europe PMC] [Abstract]
  18. "The two DNA clamps Rad9/Rad1/Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity."J. Mol. Biol. 353:980-989(2005) [PubMed] [Europe PMC] [Abstract]
  19. "Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells."Oncogene 24:4728-4735(2005) [PubMed] [Europe PMC] [Abstract]
  20. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  21. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  24. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  25. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  26. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  27. "A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling."Science 332:1313-1317(2011) [PubMed] [Europe PMC] [Abstract]
  28. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]