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Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1)

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Gnb2-rs1; H12.3; HLC-7; PIG21; RACK1; Receptor For Activated C Kinase 1; Cell proliferation-inducing gene 21 protein; Human lung cancer oncogene 7 protein

Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1)
CCC K335
HGNC 4399
MGI 101849
PubMed 19000751
RGD 69229
UniProt P63244
Wiki GNB2L1
RACK1 is a major component of translating ribosomes, which harbor significant amounts of PKC. Loading 60S subunits with EIF6 caused a dose-dependent translational block and impairment of 80S formation, which were reversed by expression of RACK1 and stimulation of PKC in vivo and in vitro. PKC stimulation led to EIF6 phosphorylation, and mutation of a serine residue in the carboxy terminus of EIF6 impaired RACK1/PKC-mediated translational rescue.
The deduced 317-amino acid protein shares significant similarity with GNB2, including an identical structure of 7 homologous segments and a number of identical or isofunctional consensus residues. Northern blot analysis detected a 1.3-kb transcript abundantly expressed in B-lymphoblastoid cells.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Guanine Nucleotide Binding Protein Subunit Beta 2 Like Protein 1 (GNb2L1) ELISA Kit Customized Service Offer
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  4. , Submitted (MAY-2006) to UniProtKB
  5. , Submitted (MAR-2007) to UniProtKB
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  7. "The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1."Eur. J. Biochem. 267:3891-3901(2000) [PubMed] [Europe PMC] [Abstract]
  8. "The interaction of Src and RACK1 is enhanced by activation of protein kinase C and tyrosine phosphorylation of RACK1."J. Biol. Chem. 276:20346-20356(2001) [PubMed] [Europe PMC] [Abstract]
  9. "Rack1 binds HIV-1 Nef and can act as a Nef-protein kinase C adaptor."Virology 283:7-18(2001) [PubMed] [Europe PMC] [Abstract]
  10. "Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor."J. Biol. Chem. 277:22925-22933(2002) [PubMed] [Europe PMC] [Abstract]
  11. "RACK1, an insulin-like growth factor I (IGF-I) receptor-interacting protein, modulates IGF-I-dependent integrin signaling and promotes cell spreading and contact with extracellular matrix."Mol. Cell. Biol. 22:2345-2365(2002) [PubMed] [Europe PMC] [Abstract]
  12. "RACK1: a novel substrate for the Src protein-tyrosine kinase."Oncogene 21:7619-7629(2002) [PubMed] [Europe PMC] [Abstract]
  13. "Alternative splicing of the human proto-oncogene c-H-ras renders a new Ras family protein that trafficks to cytoplasm and nucleus."Cancer Res. 63:5178-5187(2003) [PubMed] [Europe PMC] [Abstract]
  14. "The scaffolding protein RACK1 interacts with androgen receptor and promotes cross-talk through a protein kinase C signaling pathway."J. Biol. Chem. 278:46087-46093(2003) [PubMed] [Europe PMC] [Abstract]
  15. "RACK1 regulates integrin-mediated adhesion, protrusion, and chemotactic cell migration via its Src-binding site."Mol. Biol. Cell 14:658-669(2003) [PubMed] [Europe PMC] [Abstract]
  16. "Muscle ring finger protein-1 inhibits PKC-epsilon activation and prevents cardiomyocyte hypertrophy."J. Cell Biol. 167:1147-1159(2004) [PubMed] [Europe PMC] [Abstract]
  17. "Ki-1/57 interacts with RACK1 and is a substrate for the phosphorylation by phorbol 12-myristate 13-acetate-activated protein kinase C."J. Biol. Chem. 279:11444-11455(2004) [PubMed] [Europe PMC] [Abstract]
  18. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
  19. "Receptor for activated C kinase 1 (RACK1) and Src regulate the tyrosine phosphorylation and function of the androgen receptor."Cancer Res. 66:11047-11054(2006) [PubMed] [Europe PMC] [Abstract]
  20. "Interaction of integrin beta1 with cytokeratin 1 in neuroblastoma NMB7 cells."Biochem. Soc. Trans. 35:1292-1294(2007) [PubMed] [Europe PMC] [Abstract]
  21. "RACK1 competes with HSP90 for binding to HIF-1alpha and is required for O(2)-independent and HSP90 inhibitor-induced degradation of HIF-1alpha."Mol. Cell 25:207-217(2007) [PubMed] [Europe PMC] [Abstract]
  22. "RACK1 inhibits TRPM6 activity via phosphorylation of the fused alpha-kinase domain."Curr. Biol. 18:168-176(2008) [PubMed] [Europe PMC] [Abstract]
  23. "RACK1, a new ADAM12 interacting protein. Contribution to liver fibrogenesis."J. Biol. Chem. 283:26000-26009(2008) [PubMed] [Europe PMC] [Abstract]
  24. "RACK1 regulates the cell surface expression of the G protein-coupled receptor for thromboxane A(2)."Traffic 9:394-407(2008) [PubMed] [Europe PMC] [Abstract]
  25. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  26. "RACK1 associates with CLEC-2 and promotes its ubiquitin-proteasome degradation."Biochem. Biophys. Res. Commun. 390:217-222(2009) [PubMed] [Europe PMC] [Abstract]
  27. "Structural basis and specificity of human otubain 1-mediated deubiquitination."Biochem. J. 418:379-390(2009) [PubMed] [Europe PMC] [Abstract]
  28. "Phosphorylation of RACK1 on tyrosine 52 by c-Abl is required for insulin-like growth factor I-mediated regulation of focal adhesion kinase."J. Biol. Chem. 284:20263-20274(2009) [PubMed] [Europe PMC] [Abstract]
  29. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  30. "RACK1 promotes Bax oligomerization and dissociates the interaction of Bax and Bcl-XL."Cell. Signal. 22:1495-1501(2010) [PubMed] [Europe PMC] [Abstract]
  31. "RACK1 associates with muscarinic receptors and regulates M(2) receptor trafficking."PLoS ONE 5:E13517-E13517(2010) [PubMed] [Europe PMC] [Abstract]
  32. "A stimulatory role for the La-related protein 4B in translation."RNA 16:1488-1499(2010) [PubMed] [Europe PMC] [Abstract]
  33. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  34. "RACK1 promotes breast carcinoma migration/metastasis via activation of the RhoA/Rho kinase pathway."Breast Cancer Res. Treat. 126:555-563(2011) [PubMed] [Europe PMC] [Abstract]
  35. "RACK1 regulates VEGF/Flt1-mediated cell migration via activation of a PI3-K/Akt pathway."J. Biol. Chem. 286:9097-9106(2011) [PubMed] [Europe PMC] [Abstract]
  36. "The RACK1 signaling scaffold protein selectively interacts with Yersinia pseudotuberculosis virulence function."PLoS ONE 6:E16784-E16784(2011) [PubMed] [Europe PMC] [Abstract]
  37. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  38. "Receptor for activated C kinase 1 (RACK1) inhibits function of transient receptor potential (TRP)-type channel Pkd2L1 through physical interaction."J. Biol. Chem. 287:6551-6561(2012) [PubMed] [Europe PMC] [Abstract]
  39. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  40. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  41. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  42. "Solution structure of the human signaling protein RACK1."BMC Struct. Biol. 10:15-15(2010) [PubMed] [Europe PMC] [Abstract]
  43. "Structure of human Rack1 protein at a resolution of 2.45 A."Acta Crystallogr. F 68:867-872(2012) [PubMed] [Europe PMC] [Abstract]
  44. "Structures of the human and Drosophila 80S ribosome."Nature 497:80-85(2013) [PubMed] [Europe PMC] [Abstract]