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Decapping Enzyme 1A (DCP1A)

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SMAD4IP1; SMIF; Transcription factor SMIF; Smad4-interacting transcriptional co-activator; mRNA-decapping enzyme 1A

Decapping Enzyme 1A (DCP1A)
CCC L411
HGNC 18714
MGI 1923151
PubMed 23637887
RGD 1561298
UniProt Q9NPI6
Wiki DCP1A
DCP1A and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.Recombinant DCP1A fused to an N-terminal GST tag was inactive in decapping activity, but FLAG-tagged DCP1A expressed in human embryonic kidney cells was active. Mutation of asn20 and arg59 reduced DCP1A decapping activity by 4- and 12-fold, respectively, and both mutations resulted in reduced protein stability. Coimmunoprecipitation assays demonstrated that DCP1A and DCP2 interacted with the nonsense-mediated decay factor UPF1 (RENT1) both in the presence and in the absence of other UPF proteins.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Decapping Enzyme 1A (DCP1A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Decapping Enzyme 1A (DCP1A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Decapping Enzyme 1A (DCP1A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Decapping Enzyme 1A (DCP1A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Decapping Enzyme 1A (DCP1A) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Decapping Enzyme 1A (DCP1A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Decapping Enzyme 1A (DCP1A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Decapping Enzyme 1A (DCP1A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Decapping Enzyme 1A (DCP1A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Decapping Enzyme 1A (DCP1A) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Decapping Enzyme 1A (DCP1A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Decapping Enzyme 1A (DCP1A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Decapping Enzyme 1A (DCP1A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Decapping Enzyme 1A (DCP1A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Decapping Enzyme 1A (DCP1A) ELISA Kit Customized Service Offer
  1. "Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay."Mol. Cell. Biol. 22:8114-8121(2002) [PubMed] [Europe PMC] [Abstract]
  2. "SMIF, a Smad4-interacting protein that functions as a co-activator in TGFbeta signalling."Nat. Cell Biol. 4:181-190(2002) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence, annotation and analysis of human chromosome 3." Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci."RNA 8:1489-1501(2002) [PubMed] [Europe PMC] [Abstract]
  7. "Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping."Mol. Cell 20:905-915(2005) [PubMed] [Europe PMC] [Abstract]
  8. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  9. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  11. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Human proline-rich nuclear receptor coregulatory protein 2 mediates an interaction between mRNA surveillance machinery and decapping complex."Mol. Cell 33:75-86(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  16. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  17. "Zinc-finger antiviral protein inhibits HIV-1 infection by selectively targeting multiply spliced viral mRNAs for degradation."Proc. Natl. Acad. Sci. U.S.A. 108:15834-15839(2011) [PubMed] [Europe PMC] [Abstract]
  18. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  19. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]