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Decapping Enzyme 2 (DCP2)

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NUDT20; Nudix(Nucleoside Diphosphate Linked Moiety X)-Type Motif 20; mRNA-decapping enzyme 2; m7GpppN-mRNA hydrolase

Decapping Enzyme 2 (DCP2)
CCC L412
HGNC 24452
MGI 1917890
PubMed 12218187
RGD 1562909
UniProt Q8IU60
Wiki DCP2
DCP2 is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end .
Epitope-tagged DCP2 localized to punctate cytoplasmic foci, with some nuclear staining.The deduced protein contains 3 conserved regions: the 109-amino acid Nudix fold and its flanking A and B boxes. The Nudix motif within the fold contains 2 glutamic acids (glu147 and glu148) critical for metal ion coordination and pyrophosphatase activity. Western blot analysis detected endogenous DCP2 at an apparent molecular mass of 45 kD in cytoplasmic fractions of K562 cells. DCP2 predominantly cosedimented with polysomes.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Decapping Enzyme 2 (DCP2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Decapping Enzyme 2 (DCP2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Decapping Enzyme 2 (DCP2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Decapping Enzyme 2 (DCP2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Decapping Enzyme 2 (DCP2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Decapping Enzyme 2 (DCP2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Decapping Enzyme 2 (DCP2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Decapping Enzyme 2 (DCP2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Decapping Enzyme 2 (DCP2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Decapping Enzyme 2 (DCP2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Decapping Enzyme 2 (DCP2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Decapping Enzyme 2 (DCP2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Decapping Enzyme 2 (DCP2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Decapping Enzyme 2 (DCP2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Decapping Enzyme 2 (DCP2) ELISA Kit Customized Service Offer
  1. "Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay."Mol. Cell. Biol. 22:8114-8121(2002) [PubMed] [Europe PMC] [Abstract]
  2. "The hDcp2 protein is a mammalian mRNA decapping enzyme."Proc. Natl. Acad. Sci. U.S.A. 99:12663-12668(2002) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and comparative analysis of human chromosome 5." Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Human Dcp2: a catalytically active mRNA decapping enzyme located in specific cytoplasmic structures."EMBO J. 21:6915-6924(2002) [PubMed] [Europe PMC] [Abstract]
  7. "The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci."RNA 8:1489-1501(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities."Mol. Cell 12:675-687(2003) [PubMed] [Europe PMC] [Abstract]
  9. "Functional characterization of the mammalian mRNA decapping enzyme hDcp2."RNA 9:1138-1147(2003) [PubMed] [Europe PMC] [Abstract]
  10. "A protein interaction framework for human mRNA degradation."Genome Res. 14:1315-1323(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Cytoplasmic foci are sites of mRNA decay in human cells."J. Cell Biol. 165:31-40(2004) [PubMed] [Europe PMC] [Abstract]
  12. "Functional analysis of mRNA scavenger decapping enzymes."RNA 10:1412-1422(2004) [PubMed] [Europe PMC] [Abstract]
  13. "Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping."Mol. Cell 20:905-915(2005) [PubMed] [Europe PMC] [Abstract]
  14. "Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies."PLoS Pathog. 2:E41-E41(2006) [PubMed] [Europe PMC] [Abstract]
  15. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  16. "SSA/Ro52 autoantigen interacts with Dcp2 to enhance its decapping activity."Biochem. Biophys. Res. Commun. 370:195-199(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'."Genes Dev. 22:50-65(2008) [PubMed] [Europe PMC] [Abstract]
  18. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  19. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Multiple mRNA decapping enzymes in mammalian cells."Mol. Cell 40:423-432(2010) [PubMed] [Europe PMC] [Abstract]
  21. "LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing."Proc. Natl. Acad. Sci. U.S.A. 107:12499-12504(2010) [PubMed] [Europe PMC] [Abstract]
  22. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  23. "Zinc-finger antiviral protein inhibits HIV-1 infection by selectively targeting multiply spliced viral mRNAs for degradation."Proc. Natl. Acad. Sci. U.S.A. 108:15834-15839(2011) [PubMed] [Europe PMC] [Abstract]
  24. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  25. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]