Myeloid Associated Differentiation Marker (MYADM)

MYADM shares 75.8% overall sequence identity with mouse Myadm, with highest levels of conservation seen in the predicted transmembrane regions. Northern blot analysis detected 3.3- and 2.2-kb MYADM transcripts. Expression of the longer transcript was seen in all tissues tested except thymus; expression of the shorter transcript was seen in heart, placenta, lung, pancreas, testis, and peripheral blood leukocytes and was undetectable in all other tissues. Semiquantitative PCR assays showed that expression of MYADM was not only significantly higher in peripheral blood leukocytes than in bone marrow cells, but was also upregulated in NB4 cells (derived from a patient with acute promyelocytic leukemia) that were treated with the differentiation inducer all-trans retinoic acid (ATRA).