Zinc Finger Protein 33A (ZNF33A)

Mutation analysis indicated that the SAM domain of ZAK was required to bind ZZAPK. The deduced 848-amino acid protein contains a C2H2-type zinc finger domain and shares 93.1% identity with ZNF11B (ZNF33B) over 374 amino acids. Northern blot analysis detected ZNF33A expression in all tissues examined, with highest expression in skeletal muscle, and lowest expression in testis and colon.

The deduced 810-amino acid protein has a calculated molecular mass of 94.3 kD. ZZAPK contains an N-terminal KRAB domain, followed by a 256-amino acid linker region and 16 tandemly repeated Kruppel-type zinc fingers at its C terminus. Northern blot analysis detected variable expression of a major 5.5-kb transcript in all tissues examined.