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NUAK Family SNF1 Like Kinase 1 (NUAK1)

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ARK5; OMPHK1; NuaK1; AMP-Activated Protein Kinase Family Member 5; Omphalocele kinase 1

NUAK Family SNF1 Like Kinase 1 (NUAK1)
CCC L975
HGNC 14311
MGI 1925226
PubMed 21317932
RGD 1309956
UniProt O60285
Wiki NUAK1
The deduced 661-amino acid protein shares about 52% identity over 252 amino acids with plant SNF1-related protein kinase. NUAK1 had an apparent molecular mass of about 75 kD by SDS-PAGE. RT-PCR detected highest expression in heart and brain, followed by skeletal muscle, kidney, ovary, placenta, lung, and liver. Little to no expression was detected in other tissues examined.The phosphorylation was stimulated by AMP and did not require accessory binding proteins. Transfection of ARK5 also increased the survival of HepG2 cells exposed to glucose starvation and reduced oxygen tension. Increased cell survival was accompanied by phosphorylation of ARK5 on ser600 by AKT, which activated ARK5 kinase activity. Activated ARK5 phosphorylated ATM, which led to phosphorylation of p53.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPL975Hu01 Recombinant NUAK Family SNF1 Like Kinase 1 (NUAK1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAL975Hu01 Polyclonal Antibody to NUAK Family SNF1 Like Kinase 1 (NUAK1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for NUAK Family SNF1 Like Kinase 1 (NUAK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for NUAK Family SNF1 Like Kinase 1 (NUAK1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPL975Mu01 Recombinant NUAK Family SNF1 Like Kinase 1 (NUAK1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAL975Mu01 Polyclonal Antibody to NUAK Family SNF1 Like Kinase 1 (NUAK1) WB; IHC
Assay Kits n/a CLIA Kit for NUAK Family SNF1 Like Kinase 1 (NUAK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for NUAK Family SNF1 Like Kinase 1 (NUAK1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant NUAK Family SNF1 Like Kinase 1 (NUAK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to NUAK Family SNF1 Like Kinase 1 (NUAK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to NUAK Family SNF1 Like Kinase 1 (NUAK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for NUAK Family SNF1 Like Kinase 1 (NUAK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for NUAK Family SNF1 Like Kinase 1 (NUAK1) ELISA Kit Customized Service Offer
  1. "LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1."EMBO J. 23:833-843(2004) [PubMed] [Europe PMC] [Abstract]
  2. "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract]
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  4. "The finished DNA sequence of human chromosome 12." Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Identification of a novel protein kinase mediating Akt survival signaling to the ATM protein."J. Biol. Chem. 278:48-53(2003) [PubMed] [Europe PMC] [Abstract]
  7. "ARK5 expression in colorectal cancer and its implications for tumor progression."Am. J. Pathol. 164:987-995(2004) [PubMed] [Europe PMC] [Abstract]
  8. "ARK5 is a tumor invasion-associated factor downstream of Akt signaling."Mol. Cell. Biol. 24:3526-3535(2004) [PubMed] [Europe PMC] [Abstract]
  9. "Regulation of caspase-6 and FLIP by the AMPK family member ARK5."Oncogene 23:7067-7075(2004) [PubMed] [Europe PMC] [Abstract]
  10. "ARK5 is transcriptionally regulated by the Large-MAF family and mediates IGF-1-induced cell invasion in multiple myeloma: ARK5 as a new molecular determinant of malignant multiple myeloma."Oncogene 24:6936-6944(2005) [PubMed] [Europe PMC] [Abstract]
  11. "Overexpression of c-Maf contributes to T-cell lymphoma in both mice and human."Cancer Res. 66:812-819(2006) [PubMed] [Europe PMC] [Abstract]
  12. "NDR2 acts as the upstream kinase of ARK5 during insulin-like growth factor-1 signaling."J. Biol. Chem. 281:13915-13921(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Control of AMPK-related kinases by USP9X and atypical Lys(29)/Lys(33)-linked polyubiquitin chains."Biochem. J. 411:249-260(2008) [PubMed] [Europe PMC] [Abstract]
  14. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Regulation of ploidy and senescence by the AMPK-related kinase NUAK1."EMBO J. 29:376-386(2010) [PubMed] [Europe PMC] [Abstract]
  16. "New roles for the LKB1-NUAK pathway in controlling myosin phosphatase complexes and cell adhesion."Sci. Signal. 3:RA25-RA25(2010) [PubMed] [Europe PMC] [Abstract]
  17. "A new role of NUAK1: directly phosphorylating p53 and regulating cell proliferation."Oncogene 30:2933-2942(2011) [PubMed] [Europe PMC] [Abstract]
  18. "A mouse model uncovers LKB1 as an UVB-induced DNA damage sensor mediating CDKN1A (p21WAF1/CIP1) degradation."PLoS Genet. 10:E1004721-E1004721(2014) [PubMed] [Europe PMC] [Abstract]
  19. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]