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Protein L-Isoaspartate-O-Methyltransferase (PCMT1)

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PIMT; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase

Protein L-Isoaspartate-O-Methyltransferase (PCMT1)
CCC L997
HGNC 8728
MGI 97502
PubMed 23918616
RGD 3268
UniProt P22061
Wiki PCMT1
Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues.
These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPL997Hu01 Recombinant Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAL997Hu21 Monoclonal Antibody to Protein L-Isoaspartate-O-Methyltransferase (PCMT1) WB; IHC; ICC; IP.
PAL997Hu01 Polyclonal Antibody to Protein L-Isoaspartate-O-Methyltransferase (PCMT1) WB; IHC; ICC; IP.
Assay Kits SEL997Hu ELISA Kit for Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein L-Isoaspartate-O-Methyltransferase (PCMT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein L-Isoaspartate-O-Methyltransferase (PCMT1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein L-Isoaspartate-O-Methyltransferase (PCMT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein L-Isoaspartate-O-Methyltransferase (PCMT1) ELISA Kit Customized Service Offer
  1. "Sequence of the D-aspartyl/L-isoaspartyl protein methyltransferase from human erythrocytes. Common sequence motifs for protein, DNA, RNA, and small molecule S-adenosylmethionine-dependent methyltransferases."J. Biol. Chem. 264:20131-20139(1989) [PubMed] [Europe PMC] [Abstract]
  2. "Alternative splicing of the human isoaspartyl protein carboxyl methyltransferase RNA leads to the generation of a C-terminal -RDEL sequence in isozyme II."Biochem. Biophys. Res. Commun. 185:277-283(1992) [PubMed] [Europe PMC] [Abstract]
  3. "Characterization of three cDNAs encoding two isozymes of an isoaspartyl protein carboxyl methyltransferase from human erythroid leukemia cells."J. Biochem. 117:683-685(1995) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and analysis of human chromosome 6." Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Structure of the human gene encoding the protein repair L-isoaspartyl (D-aspartyl) O-methyltransferase."Arch. Biochem. Biophys. 335:321-332(1996) [PubMed] [Europe PMC] [Abstract]
  8. "Purification of homologous protein carboxyl methyltransferase isozymes from human and bovine erythrocytes."Biochemistry 27:5227-5233(1988) [PubMed] [Europe PMC] [Abstract]
  9. , Submitted (DEC-2008) to UniProtKB
  10. "Amino acid polymorphisms of the human L-isoaspartyl/D-aspartyl methyltransferase involved in protein repair."Biochem. Biophys. Res. Commun. 203:491-497(1994) [PubMed] [Europe PMC] [Abstract]
  11. "Distinct C-terminal sequences of isozymes I and II of the human erythrocyte L-isoaspartyl/D-aspartyl protein methyltransferase."Biochem. Biophys. Res. Commun. 175:351-358(1991) [PubMed] [Europe PMC] [Abstract]
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  13. "Polymorphic forms of the protein L-isoaspartate (D-aspartate) O-methyltransferase involved in the repair of age-damaged proteins."J. Hum. Genet. 44:275-288(1999) [PubMed] [Europe PMC] [Abstract]
  14. "Crystal structure of human L-isoaspartyl methyltransferase."J. Biol. Chem. 277:10642-10646(2002) [PubMed] [Europe PMC] [Abstract]
  15. "Crystal structure of human L-isoaspartyl-O-methyl-transferase with S-adenosyl homocysteine at 1.6-A resolution and modeling of an isoaspartyl-containing peptide at the active site."Protein Sci. 11:625-635(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]