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Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa)

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PKACA

Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa)
CCC M062
HGNC 9380
MGI 97592
PubMed 24700472
RGD 3389
UniProt P17612
Wiki PRKACA
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the AMP-activated protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans.
PRKACais a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPM062Hu01 Recombinant Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) Polyclonal Antibody Customized Service Offer
Assay Kits SEM062Hu ELISA Kit for Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) ELISA Kit Customized Service Offer
  1. "A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha."Nucleic Acids Res. 16:8189-8190(1988) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "A novel isoform of human cyclic 3',5'-adenosine monophosphate-dependent protein kinase, c alpha-s, localizes to sperm midpiece."Biol. Reprod. 63:607-611(2000) [PubMed] [Europe PMC] [Abstract]
  5. "The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative C-alpha mRNA expressed specifically in spermatogenic cells."Mol. Biol. Cell 11:3031-3044(2000) [PubMed] [Europe PMC] [Abstract]
  6. "Expression of a nonmyristylated variant of the catalytic subunit of protein kinase A during male germ-cell development."Proc. Natl. Acad. Sci. U.S.A. 97:6433-6438(2000) [PubMed] [Europe PMC] [Abstract]
  7. "Phosphorylation of the catalytic subunit of protein kinase A. Autophosphorylation versus phosphorylation by phosphoinositide-dependent kinase-1."J. Biol. Chem. 277:47878-47884(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Rab13 regulates PKA signaling during tight junction assembly."J. Cell Biol. 165:175-180(2004) [PubMed] [Europe PMC] [Abstract]
  9. "DNA binding of repressor nuclear factor-kappaB p50/p50 depends on phosphorylation of Ser337 by the protein kinase A catalytic subunit."J. Biol. Chem. 280:9957-9962(2005) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphorylation of claudin-3 at threonine 192 by cAMP-dependent protein kinase regulates tight junction barrier function in ovarian cancer cells."J. Biol. Chem. 280:26233-26240(2005) [PubMed] [Europe PMC] [Abstract]
  11. "PKA-mediated phosphorylation regulates the function of activation-induced deaminase (AID) in B cells."Proc. Natl. Acad. Sci. U.S.A. 103:395-400(2006) [PubMed] [Europe PMC] [Abstract]
  12. "Proteasome function is regulated by cyclic AMP-dependent protein kinase through phosphorylation of Rpt6."J. Biol. Chem. 282:22460-22471(2007) [PubMed] [Europe PMC] [Abstract]
  13. "Functional consequence of protein kinase A-dependent phosphorylation of the cardiac ryanodine receptor: sensitization of store overload-induced Ca2+ release."J. Biol. Chem. 282:30256-30264(2007) [PubMed] [Europe PMC] [Abstract]
  14. "Vitamin K2 induces phosphorylation of protein kinase A and expression of novel target genes in osteoblastic cells."J. Mol. Endocrinol. 39:239-247(2007) [PubMed] [Europe PMC] [Abstract]
  15. "Activation of protein kinase A (PKA) signaling mitigates the antiproliferative and antiinvasive effects of alpha-difluoromethylornithine in breast cancer cells."Breast Cancer Res. Treat. 107:63-70(2008) [PubMed] [Europe PMC] [Abstract]
  16. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif."Nat. Struct. Mol. Biol. 15:1184-1191(2008) [PubMed] [Europe PMC] [Abstract]
  18. "Allosteric cooperativity in protein kinase A."Proc. Natl. Acad. Sci. U.S.A. 105:506-511(2008) [PubMed] [Europe PMC] [Abstract]
  19. "Gravin dynamics regulates the subcellular distribution of PKA."Exp. Cell Res. 315:1247-1259(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Thrombin and collagen induce a feedback inhibitory signaling pathway in platelets involving dissociation of the catalytic subunit of protein kinase A from an NFkappaB-IkappaB complex."J. Biol. Chem. 285:18352-18363(2010) [PubMed] [Europe PMC] [Abstract]
  23. "High glucose stimulates adipogenic and inhibits osteogenic differentiation in MG-63 cells through cAMP/protein kinase A/extracellular signal-regulated kinase pathway."Mol. Cell. Biochem. 338:115-122(2010) [PubMed] [Europe PMC] [Abstract]
  24. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  25. "Protein kinase A activates and phosphorylates ROR?4 in vitro and takes part in ROR? activation by CaMK-IV."Biochem. Biophys. Res. Commun. 408:442-446(2011) [PubMed] [Europe PMC] [Abstract]
  26. "The testis-specific C?2 subunit of PKA is kinetically indistinguishable from the common C?1 subunit of PKA."BMC Biochem. 12:40-40(2011) [PubMed] [Europe PMC] [Abstract]
  27. "Control of PKA stability and signalling by the RING ligase praja2."Nat. Cell Biol. 13:412-422(2011) [PubMed] [Europe PMC] [Abstract]
  28. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  29. "Recurrent activating mutation in PRKACA in cortisol-producing adrenal tumors."Nat. Genet. 46:613-617(2014) [PubMed] [Europe PMC] [Abstract]
  30. "Discovery of 2-pyrimidyl-5-amidothiophenes as potent inhibitors for AKT: synthesis and SAR studies."Bioorg. Med. Chem. Lett. 16:4163-4168(2006) [PubMed] [Europe PMC] [Abstract]
  31. "Azole-based inhibitors of AKT/PKB for the treatment of cancer." Bioorg. Med. Chem. Lett. 20:1559-1564(2010) [PubMed] [Europe PMC] [Abstract]
  32. "Design of selective, ATP-competitive inhibitors of Akt." J. Med. Chem. 53:4615-4622(2010) [PubMed] [Europe PMC] [Abstract]
  33. "Diversity of bisubstrate binding modes of adenosine analogue-oligoarginine conjugates in protein kinase a and implications for protein substrate interactions."J. Mol. Biol. 403:66-77(2010) [PubMed] [Europe PMC] [Abstract]
  34. "Mutants of protein kinase A that mimic the ATP-binding site of Aurora kinase."Biochem. J. 440:85-93(2011) [PubMed] [Europe PMC] [Abstract]
  35. "Experimental active site mapping as a starting point to fragment-based lead discovery."Submitted (JUL-2010) to the PDB data bank
  36. "Fragment based drug design on PKA."Submitted (SEP-2010) to the PDB data bank
  37. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
  38. "Constitutive activation of PKA catalytic subunit in adrenal Cushing's syndrome." N. Engl. J. Med. 370:1019-1028(2014) [PubMed] [Europe PMC] [Abstract]
  39. "Activating hotspot L205R mutation in PRKACA and adrenal Cushing's syndrome." Science 344:913-917(2014) [PubMed] [Europe PMC] [Abstract]
  40. "Recurrent somatic mutations underlie corticotropin-independent Cushing's syndrome." Science 344:917-920(2014) [PubMed] [Europe PMC] [Abstract]