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Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA)

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HSD14; PACT; RAX; Protein Activator Of The Interferon-Induced Protein Kinase; Protein kinase, interferon-inducible double-stranded RNA-dependent activator

Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA)
CCC M067
HGNC 9438
MGI 1344375
PubMed 18420150
RGD 1306707
UniProt O75569
Wiki PRKRA
The double-stranded RNA (dsRNA)-activated protein kinase PKR is a mediator of the effects of interferon, particularly its antiviral activities. PKR has also been implicated in the onset of differentiation.The PACT cDNA encodes a deduced 313-amino acid polypeptide that contains 3 motifs resembling the dimerization motifs present in PKR and other dsRNA-binding proteins. Northern blot analysis revealed that PACT is expressed as a 2.0-kb mRNA in a variety of human cell lines.
Northern blot analysis detected expression of RAX in all tissues tested. Western blot analysis showed a 35-kD protein expressed in cell lines derived from several mammalian species.PACT binds to both PKR and dsRNA. PACT activates PKR in a dsRNA-independent manner.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) CLIA Kit Customized Service Offer
n/a ELISA Kit for Protein Kinase, Interferon Inducible Double Stranded RNA Dependent Activator (PRKRA) ELISA Kit Customized Service Offer
  1. "PACT, a protein activator of the interferon-induced protein kinase, PKR."EMBO J. 17:4379-4390(1998) [PubMed] [Europe PMC] [Abstract]
  2. "RAX, a cellular activator for double-stranded RNA-dependent protein kinase during stress signaling."J. Biol. Chem. 274:15427-15432(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Modular structure of PACT: distinct domains for binding and activating PKR."Mol. Cell. Biol. 21:1908-1920(2001) [PubMed] [Europe PMC] [Abstract]
  8. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  9. "The role of PACT in the RNA silencing pathway."EMBO J. 25:522-532(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphorylation of specific serine residues in the PKR activation domain of PACT is essential for its ability to mediate apoptosis."J. Biol. Chem. 281:35129-35136(2006) [PubMed] [Europe PMC] [Abstract]
  11. "Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA."J. Biol. Chem. 282:17649-17657(2007) [PubMed] [Europe PMC] [Abstract]
  12. "Interaction of human tRNA-dihydrouridine synthase-2 with interferon-induced protein kinase PKR."Nucleic Acids Res. 36:998-1008(2008) [PubMed] [Europe PMC] [Abstract]
  13. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  14. "Interactions between the double-stranded RNA-binding proteins TRBP and PACT define the Medipal domain that mediates protein-protein interactions."RNA Biol. 5:92-103(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  16. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  18. "The RAX/PACT-PKR stress response pathway promotes p53 sumoylation and activation, leading to G(1) arrest."Cell Cycle 11:407-417(2012) [PubMed] [Europe PMC] [Abstract]
  19. "Solution structure of the DSRM domain of protein activator of the interferon-induced protein kinase."Submitted (SEP-2006) to the PDB data bank
  20. "DYT16, a novel young-onset dystonia-parkinsonism disorder: identification of a segregating mutation in the stress-response protein PRKRA."Lancet Neurol. 7:207-215(2008) [PubMed] [Europe PMC] [Abstract]
  21. "A heterozygous frameshift mutation in PRKRA (DYT16) associated with generalised dystonia in a German patient."Lancet Neurol. 7:380-381(2008) [PubMed] [Europe PMC] [Abstract]