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Positive Cofactor 4 (PC4)

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SUB1; p14; P15; Activated RNA Polymerase II Transcriptional Coactivator p15

Positive Cofactor 4 (PC4)
CCC M320
HGNC 19985
MGI 104811
PubMed 19047459
RGD 621582
UniProt P53999
The latter include TBP-associated factors (TAFs), which interact with the activation domains of various regulatory proteins to enhance transcription. A second group of positive cofactors includes PC1, PC2, and PC3; they are distinct from TFIID and are present in the so-called upstream stimulatory activity (USA) cell fraction. These factors also have a strong positive influence on transcription. A third group of factors isolated from the USA fraction suppress transcription.
The 127-amino acid protein was demonstrated to be a single-stranded DNA-binding protein and the recombinant PC4 was shown to function identically to the native protein through its interaction with TAFs. Protein kinase CK2, in conjunction with the coactivator PC4, established DPE-specific transcription.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Positive Cofactor 4 (PC4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Positive Cofactor 4 (PC4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Positive Cofactor 4 (PC4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Positive Cofactor 4 (PC4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Positive Cofactor 4 (PC4) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Positive Cofactor 4 (PC4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Positive Cofactor 4 (PC4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Positive Cofactor 4 (PC4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Positive Cofactor 4 (PC4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Positive Cofactor 4 (PC4) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Positive Cofactor 4 (PC4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Positive Cofactor 4 (PC4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Positive Cofactor 4 (PC4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Positive Cofactor 4 (PC4) CLIA Kit Customized Service Offer
n/a ELISA Kit for Positive Cofactor 4 (PC4) ELISA Kit Customized Service Offer
  1. "A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators."Cell 78:525-534(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes."Cell 78:513-523(1994) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Phosphorylation negatively regulates the function of coactivator PC4."Proc. Natl. Acad. Sci. U.S.A. 91:12691-12695(1994) [PubMed] [Europe PMC] [Abstract]
  5. "The coactivator p15 (PC4) initiates transcriptional activation during TFIIA-TFIID-promoter complex formation."EMBO J. 14:3520-3527(1995) [PubMed] [Europe PMC] [Abstract]
  6. "A dynamic model for PC4 coactivator function in RNA polymerase II transcription."Proc. Natl. Acad. Sci. U.S.A. 95:2192-2197(1998) [PubMed] [Europe PMC] [Abstract]
  7. "Evolutionarily conserved interaction between CstF-64 and PC4 links transcription, polyadenylation, and termination."Mol. Cell 7:1013-1023(2001) [PubMed] [Europe PMC] [Abstract]
  8. "Gradual phosphorylation regulates PC4 coactivator function."FEBS J. 273:1430-1444(2006) [PubMed] [Europe PMC] [Abstract]
  9. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  15. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  16. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  17. "C-terminal domain of transcription cofactor PC4 reveals dimeric ssDNA binding site."Nat. Struct. Biol. 4:900-903(1997) [PubMed] [Europe PMC] [Abstract]
  18. "The intrinsically unstructured domain of PC4 modulates the activity of the structured core through inter- and intramolecular interactions."Biochemistry 45:5067-5081(2006) [PubMed] [Europe PMC] [Abstract]
  19. "A global transcription cofactor bound to juxtaposed strands of unwound DNA."Nat. Struct. Biol. 13:181-182(2006) [PubMed] [Europe PMC] [Abstract]