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Suppressor Of Ty 5 Homolog (SUPT5H)

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SPT5; SPT5H; DRB sensitivity-inducing factor 160 kDa subunit; DRB sensitivity-inducing factor large subunit; Tat-cotransactivator 1 protein; Transcription elongation factor SPT5

Suppressor Of Ty 5 Homolog (SUPT5H)
CCC M324
HGNC 11469
MGI 1202400
PubMed 8975720
RGD 1563807
UniProt O00267
Wiki SUPT5H
The yeast suppressor of Ty (SPT) genes were isolated based on their ability to suppress insertion of the Ty phage. These genes encode a variety of proteins that are involved in chromosome activity, including histones and transcription factors. The SPT genes are not necessarily homologs of one another. SPT4, SPT5, and SPT6 are believed to be parts of a protein complex involved in transcriptional repression by modulating chromatin structure.
The N terminus of SUPT5H is highly acidic, while the C terminus contains 7 mitogen-activated protein (MAP) kinase consensus sequences.the SUPT5H sequence contains a putative nuclear localization signal. SUPT5H was expressed as a 4-kb transcript at varying levels in every adult human tissue tested.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
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Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Suppressor Of Ty 5 Homolog (SUPT5H) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Suppressor Of Ty 5 Homolog (SUPT5H) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Suppressor Of Ty 5 Homolog (SUPT5H) Polyclonal Antibody Customized Service Offer
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Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Suppressor Of Ty 5 Homolog (SUPT5H) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Suppressor Of Ty 5 Homolog (SUPT5H) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Suppressor Of Ty 5 Homolog (SUPT5H) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Suppressor Of Ty 5 Homolog (SUPT5H) CLIA Kit Customized Service Offer
n/a ELISA Kit for Suppressor Of Ty 5 Homolog (SUPT5H) ELISA Kit Customized Service Offer
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  2. "Human Supt5h protein, a putative modulator of chromatin structure, is reversibly phosphorylated in mitosis."FEBS Lett. 409:74-78(1997) [PubMed] [Europe PMC] [Abstract]
  3. "DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs."Genes Dev. 12:343-356(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation."J. Mol. Biol. 277:179-197(1998) [PubMed] [Europe PMC] [Abstract]
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  6. "Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro."EMBO J. 17:7395-7403(1998) [PubMed] [Europe PMC] [Abstract]
  7. "NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation."Cell 97:41-51(1999) [PubMed] [Europe PMC] [Abstract]
  8. "A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription."EMBO J. 18:3688-3701(1999) [PubMed] [Europe PMC] [Abstract]
  9. "Transcription elongation factor hSPT5 stimulates mRNA capping."Genes Dev. 13:1774-1779(1999) [PubMed] [Europe PMC] [Abstract]
  10. "Structure and function of the human transcription elongation factor DSIF."J. Biol. Chem. 274:8085-8092(1999) [PubMed] [Europe PMC] [Abstract]
  11. "Tat-SF1 protein associates with RAP30 and human SPT5 proteins."Mol. Cell. Biol. 19:5960-5968(1999) [PubMed] [Europe PMC] [Abstract]
  12. "FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH."Mol. Cell 5:1067-1072(2000) [PubMed] [Europe PMC] [Abstract]
  13. "Domains in the SPT5 protein that modulate its transcriptional regulatory properties."Mol. Cell. Biol. 20:2970-2983(2000) [PubMed] [Europe PMC] [Abstract]
  14. "Positive transcription elongation factor B phosphorylates hSPT5 and RNA polymerase II carboxyl-terminal domain independently of cyclin-dependent kinase-activating kinase."J. Biol. Chem. 276:12317-12323(2001) [PubMed] [Europe PMC] [Abstract]
  15. "DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation."J. Biol. Chem. 276:12951-12958(2001) [PubMed] [Europe PMC] [Abstract]
  16. "A highly purified RNA polymerase II elongation control system."J. Biol. Chem. 276:42601-42609(2001) [PubMed] [Europe PMC] [Abstract]
  17. "The peptidyl-prolyl isomerase Pin1 interacts with hSpt5 phosphorylated by Cdk9."J. Mol. Biol. 312:675-685(2001) [PubMed] [Europe PMC] [Abstract]
  18. "Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences."Mol. Cell. Biol. 22:1079-1093(2002) [PubMed] [Europe PMC] [Abstract]
  19. "Evidence that negative elongation factor represses transcription elongation through binding to a DRB sensitivity-inducing factor/RNA polymerase II complex and RNA."Mol. Cell. Biol. 22:2918-2927(2002) [PubMed] [Europe PMC] [Abstract]
  20. "Structure-function analysis of human Spt4: evidence that hSpt4 and hSpt5 exert their roles in transcriptional elongation as parts of the DSIF complex."Genes Cells 8:371-378(2003) [PubMed] [Europe PMC] [Abstract]
  21. "Methylation of SPT5 regulates its interaction with RNA polymerase II and transcriptional elongation properties."Mol. Cell 11:1055-1066(2003) [PubMed] [Europe PMC] [Abstract]
  22. "Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex."Mol. Cell. Biol. 23:1863-1873(2003) [PubMed] [Europe PMC] [Abstract]
  23. "Locus-specific requirements for Spt5 in transcriptional activation and repression in Drosophila."Curr. Biol. 14:1680-1684(2004) [PubMed] [Europe PMC] [Abstract]
  24. "Coordination of transcription factor phosphorylation and histone methylation by the P-TEFb kinase during human immunodeficiency virus type 1 transcription."J. Virol. 78:13522-13533(2004) [PubMed] [Europe PMC] [Abstract]
  25. "Dynamics of human immunodeficiency virus transcription: P-TEFb phosphorylates RD and dissociates negative effectors from the transactivation response element."Mol. Cell. Biol. 24:787-795(2004) [PubMed] [Europe PMC] [Abstract]
  26. "Human Spt6 stimulates transcription elongation by RNA polymerase II in vitro."Mol. Cell. Biol. 24:3324-3336(2004) [PubMed] [Europe PMC] [Abstract]
  27. "Functional interactions of RNA-capping enzyme with factors that positively and negatively regulate promoter escape by RNA polymerase II."Proc. Natl. Acad. Sci. U.S.A. 101:7572-7577(2004) [PubMed] [Europe PMC] [Abstract]
  28. "A negative elongation factor for human RNA polymerase II inhibits the anti-arrest transcript-cleavage factor TFIIS."Proc. Natl. Acad. Sci. U.S.A. 102:15036-15041(2005) [PubMed] [Europe PMC] [Abstract]
  29. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  30. "Dichotomous but stringent substrate selection by the dual-function Cdk7 complex revealed by chemical genetics."Nat. Struct. Mol. Biol. 13:55-62(2006) [PubMed] [Europe PMC] [Abstract]
  31. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  32. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  33. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  34. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  35. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  36. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  37. "Solution structure of KOW motifs of human transcription elongation factor SPT5."Submitted (JUL-2007) to the PDB data bank
  38. "Crystal structure of the human transcription elongation factor DSIF hSpt4 subunit in complex with the hSpt5 dimerization interface."Biochem. J. 425:373-380(2010) [PubMed] [Europe PMC] [Abstract]