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TRAF2 And NCK Interacting Protein Kinase (TNIK)

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TRAF2 And NCK Interacting Protein Kinase (TNIK)
CCC M381
HGNC 30765
MGI 1916264
PubMed 22904686
RGD 1561817
UniProt Q9UKE5
Wiki TNIK
TNIK was autophosphorylated in a manner dependent upon lys54 in the ATP-binding pocket of its kinase domain. Immunoprecipitation analysis showed that epitope-tagged TNIK coprecipitated endogenous TRAF2 from human embryonic kidney cells. Mutation analysis revealed that the intermediate domain was sufficient for the interaction, although the GCK domain may contribute.
The intermediate domain was also sufficient for interaction with NCK. Cotransfection of TNIK with JNK2 (MAPK9) enhanced JNK2 kinase activity in a dose-dependent manner, and this effect was mediated by the GCK region of TNIK, but not the kinase domain. TNIK overexpression had no effect on ERK1 (MAPK3), p38 (MAPK14), or NF-kappa-B.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant TRAF2 And NCK Interacting Protein Kinase (TNIK) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to TRAF2 And NCK Interacting Protein Kinase (TNIK) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to TRAF2 And NCK Interacting Protein Kinase (TNIK) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for TRAF2 And NCK Interacting Protein Kinase (TNIK) CLIA Kit Customized Service Offer
n/a ELISA Kit for TRAF2 And NCK Interacting Protein Kinase (TNIK) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant TRAF2 And NCK Interacting Protein Kinase (TNIK) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to TRAF2 And NCK Interacting Protein Kinase (TNIK) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to TRAF2 And NCK Interacting Protein Kinase (TNIK) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for TRAF2 And NCK Interacting Protein Kinase (TNIK) CLIA Kit Customized Service Offer
n/a ELISA Kit for TRAF2 And NCK Interacting Protein Kinase (TNIK) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant TRAF2 And NCK Interacting Protein Kinase (TNIK) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to TRAF2 And NCK Interacting Protein Kinase (TNIK) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to TRAF2 And NCK Interacting Protein Kinase (TNIK) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for TRAF2 And NCK Interacting Protein Kinase (TNIK) CLIA Kit Customized Service Offer
n/a ELISA Kit for TRAF2 And NCK Interacting Protein Kinase (TNIK) ELISA Kit Customized Service Offer
  1. "TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton."J. Biol. Chem. 274:30729-30737(1999) [PubMed] [Europe PMC] [Abstract]
  2. "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."DNA Res. 5:31-39(1998) [PubMed] [Europe PMC] [Abstract]
  3. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence, annotation and analysis of human chromosome 3." Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
  8. "The Traf2- and Nck-interacting kinase as a putative effector of Rap2 to regulate actin cytoskeleton."J. Biol. Chem. 279:49488-49496(2004) [PubMed] [Europe PMC] [Abstract]
  9. "MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1."Biochem. Biophys. Res. Commun. 377:573-578(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  12. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Rap2 function requires palmitoylation and recycling endosome localization."Biochem. Biophys. Res. Commun. 378:732-737(2009) [PubMed] [Europe PMC] [Abstract]
  15. "The kinase TNIK is an essential activator of Wnt target genes."EMBO J. 28:3329-3340(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Regulation of Rap2A by the ubiquitin ligase Nedd4-1 controls neurite development."Neuron 65:358-372(2010) [PubMed] [Europe PMC] [Abstract]
  18. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  19. "Smad inhibition by the Ste20 kinase Misshapen."Proc. Natl. Acad. Sci. U.S.A. 108:11127-11132(2011) [PubMed] [Europe PMC] [Abstract]
  20. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  21. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  22. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]