Golgin A8 Family, Member B (GOLGA8B)

By database analysis with the sequence of the Golgi complex autoantigen golgin-95/golgin-130 (GOLGA2) as probe, Eystathioy et al. (2000) identified a novel Golgi autoantigen, golgin-67. Using overlapping cDNAs and RACE, Eystathioy et al. (2000) constructed a full-length clone encoding a 459-amino acid protein with numerous alpha-helical coiled-coils.

Golgin-67 shares 4 regions with sequence identity to golgin-95, ranging between 42% and 60%. Of 84 human anti-Golgi sera, Eystathioy et al. (2000) identified 5 (6%) containing autoantibodies to golgin-67. Two of these sera, from patients with Sjogren syndrome and rheumatoid arthritis , bound to a protein of 67 kD on Western blots. Anti-golgin-67 human sera and affinity purified rabbit antibody to the recombinant protein gave predominant Golgi staining.