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Ring Finger Protein 168 (RNF168)

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E3 ubiquitin-protein ligase RNF168

Ring Finger Protein 168 (RNF168)
CCC N067
HGNC 26661
MGI 1917488
PubMed 22980979
UniProt Q8IYW5
The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones. RNF168 is an E3 ubiquitin ligase critical for DSB repair.
RNF168 was required to sustain conjugated ubiquitin at DSBs. RNF168 assembled at DSBs in an RNF8 -dependent manner and functioned as an E3 ubiquitin ligase that acted with UBC13 (UBE2N) to catalyze formation of lys63-linked ubiquitin conjugates and promote ubiquitination of H2A and H2AX (H2AFX). Ubiquitinated H2A and H2AX in turn mediated accumulation of 53BP1 and BRCA1 at DSBs to promote DSB repair and DNA damage checkpoints. The deduced 571-amino acid protein contains an N-terminal RING finger domain, followed by 2 'motifs interacting with ubiquitin' (MIU) domains.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ring Finger Protein 168 (RNF168) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ring Finger Protein 168 (RNF168) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ring Finger Protein 168 (RNF168) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ring Finger Protein 168 (RNF168) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ring Finger Protein 168 (RNF168) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ring Finger Protein 168 (RNF168) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ring Finger Protein 168 (RNF168) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ring Finger Protein 168 (RNF168) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ring Finger Protein 168 (RNF168) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ring Finger Protein 168 (RNF168) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ring Finger Protein 168 (RNF168) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ring Finger Protein 168 (RNF168) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ring Finger Protein 168 (RNF168) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ring Finger Protein 168 (RNF168) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ring Finger Protein 168 (RNF168) ELISA Kit Customized Service Offer
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  7. "RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins."Cell 136:435-446(2009) [PubMed] [Europe PMC] [Abstract]
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  12. "RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites."EMBO J. 31:1865-1878(2012) [PubMed] [Europe PMC] [Abstract]
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  16. "RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA Damage signaling."Cell 150:1182-1195(2012) [PubMed] [Europe PMC] [Abstract]
  17. "Molecular insights into the function of RING Finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation."J. Biol. Chem. 287:23900-23910(2012) [PubMed] [Europe PMC] [Abstract]