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Rho GTPase Activating Protein 21 (ARHGAP21)

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ARHGAP10

Rho GTPase Activating Protein 21 (ARHGAP21)
CCC N393
HGNC 23725
MGI 1918685
RGD 1311028
UniProt Q5T5U3
ARHGAP21 functions preferentially as a GTPase-activating protein (GAP) for CDC42 and regulates the ARP2/3 complex and F-actin dynamics at the Golgi through control of CDC42 activity.
GST pull-down and mutation analyses showed that the interactions occurred through the PH domain of ARHGAP21. RHOGAP assays indicated that ARHGAP21 acted preferentially on CDC42 over RHOA (ARHA) and RAC1. Small interfering (siRNA) treatment of ARHGAP21 led to constitutive activation of CDC42 that resulted in actin cytoskeleton remodeling, suggesting that ARHGAP21 is required for Golgi complex organization. Loss of ARHGAP21 also caused a massive activation/recruitment of the ARP2/3 complex in the juxtanuclear region.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Rho GTPase Activating Protein 21 (ARHGAP21) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Rho GTPase Activating Protein 21 (ARHGAP21) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Rho GTPase Activating Protein 21 (ARHGAP21) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Rho GTPase Activating Protein 21 (ARHGAP21) CLIA Kit Customized Service Offer
n/a ELISA Kit for Rho GTPase Activating Protein 21 (ARHGAP21) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Rho GTPase Activating Protein 21 (ARHGAP21) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Rho GTPase Activating Protein 21 (ARHGAP21) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Rho GTPase Activating Protein 21 (ARHGAP21) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Rho GTPase Activating Protein 21 (ARHGAP21) CLIA Kit Customized Service Offer
n/a ELISA Kit for Rho GTPase Activating Protein 21 (ARHGAP21) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Rho GTPase Activating Protein 21 (ARHGAP21) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Rho GTPase Activating Protein 21 (ARHGAP21) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Rho GTPase Activating Protein 21 (ARHGAP21) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Rho GTPase Activating Protein 21 (ARHGAP21) CLIA Kit Customized Service Offer
n/a ELISA Kit for Rho GTPase Activating Protein 21 (ARHGAP21) ELISA Kit Customized Service Offer
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  4. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
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  8. "Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics."Nat. Cell Biol. 7:353-364(2005) [PubMed] [Europe PMC] [Abstract]
  9. "ARHGAP10 is necessary for alpha-catenin recruitment at adherens junctions and for Listeria invasion."Nat. Cell Biol. 7:954-960(2005) [PubMed] [Europe PMC] [Abstract]
  10. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  11. "Role of the Arf6 GDP/GTP cycle and Arf6 GTPase-activating proteins in actin remodeling and intracellular transport."J. Biol. Chem. 281:12352-12361(2006) [PubMed] [Europe PMC] [Abstract]
  12. "Head and neck squamous cell carcinoma transcriptome analysis by comprehensive validated differential display."Oncogene 25:1821-1831(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  14. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  18. "Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3."FEBS Lett. 586:3522-3528(2012) [PubMed] [Europe PMC] [Abstract]
  19. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  20. "Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes."EMBO J. 26:1953-1962(2007) [PubMed] [Europe PMC] [Abstract]
  21. "Solution structure of the PH domain of Rho GTPase-activating protein 21 from human."Submitted (SEP-2006) to the PDB data bank