≡

Activator Of S-Phase Kinase (ASK)

[Edit]

DBF4; DBF4A; Chif; ZDBF1; Chiffon Homolog; Zinc Finger,DBF-Type Containing 1

Activator Of S-Phase Kinase (ASK)
CCC N854
HGNC 17364
MGI 1351328
PubMed 15374998
RGD 1305854
UniProt Q9UBU7
ASK interacts with CDC7L1 in mammalian cells and that these proteins form a complex in vivo. The authors showed that motif C of ASK is essential but not sufficient for the interaction of ASK with the CDC7L1 catalytic subunit. In an in vitro assay, ASK stimulated the kinase activity of CDC7L1, with the ASK and CDC7L1 complex capable of phosphorylating MCM2. Immunodepletion of ASK protein from cell extracts was accompanied by loss of nearly all CDC7L1-dependent kinase activity, suggesting that ASK is the major activator of CDC7L1. Expression of ASK was regulated by growth factor stimulation, with the mRNA level low during the quiescent state and increasing as the cell entered S phase. ASK protein was extensively phosphorylated during S phase, presumably by CDC7L1.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Activator Of S-Phase Kinase (ASK) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Activator Of S-Phase Kinase (ASK) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Activator Of S-Phase Kinase (ASK) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Activator Of S-Phase Kinase (ASK) CLIA Kit Customized Service Offer
n/a ELISA Kit for Activator Of S-Phase Kinase (ASK) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Activator Of S-Phase Kinase (ASK) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Activator Of S-Phase Kinase (ASK) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Activator Of S-Phase Kinase (ASK) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Activator Of S-Phase Kinase (ASK) CLIA Kit Customized Service Offer
n/a ELISA Kit for Activator Of S-Phase Kinase (ASK) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Activator Of S-Phase Kinase (ASK) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Activator Of S-Phase Kinase (ASK) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Activator Of S-Phase Kinase (ASK) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Activator Of S-Phase Kinase (ASK) CLIA Kit Customized Service Offer
n/a ELISA Kit for Activator Of S-Phase Kinase (ASK) ELISA Kit Customized Service Offer
  1. "A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition in mammalian cells."Mol. Cell. Biol. 19:5083-5095(1999) [PubMed] [Europe PMC] [Abstract]
  2. "Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication."EMBO J. 18:5703-5713(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence of human chromosome 7." Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
  5. "Human chromosome 7: DNA sequence and biology." Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Functional interaction between tumor suppressor menin and activator of S-phase kinase."Cancer Res. 64:6791-6796(2004) [PubMed] [Europe PMC] [Abstract]
  8. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  9. "Cdc7 is an active kinase in human cancer cells undergoing replication stress."J. Biol. Chem. 282:208-215(2007) [PubMed] [Europe PMC] [Abstract]
  10. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  11. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  12. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]