Pleckstrin And Sec7 Domain Containing Protein (PSD)

PSD contains an open reading frame encoding a putative protein of 645 amino acids with a predicted molecular weight of 71 kD. Database homology searches indicated that the novel gene codes for a putative protein containing 2 discrete domains with significant homology to the Sec7 and pleckstrin-homology (PH) domains, respectively.' Northern blot analysis of a panel of RNAs from normal human tissues using the PSD cDNA as probe revealed the presence of 3 different tissue-specific transcripts of approximately 4.3, 2.3, and 1.8 kb, the longest of which was expressed only in brain. The data suggested that the PSD gene may encode a protein related to the protein family containing both the Sec7 in the PH domains and thought to be involved in signaling transduction processes.