≡

Nuclear Protein, Ataxia Telangiectasia Locus (NPAT)

[Edit]

E14; p220; CAND3; Nuclear protein of the ataxia telangiectasia mutated locus

Nuclear Protein, Ataxia Telangiectasia Locus (NPAT)
CCC P831
HGNC 7896
MGI 107605
PubMed 10995386
RGD 1311947
UniProt Q14207
Wiki NPAT
NPAT lies only 0.5 kb from the 5-prime end of the ATM gene and is transcribed in the opposite direction as ATM.NPAT encodes a 1,427-amino acid protein containing nuclear localization signals and target sites for phosphorylation by cyclin-dependent protein kinases associated with E2F. NPAT has a calculated molecular mass of 154,300 Da. It is relatively serine and threonine rich. The mRNA of NPAT was detected in all human tissues examined and its genomic sequence was strongly conserved through eukaryotes, suggesting that the NPAT gene may be essential for cell maintenance, i.e., a housekeeping gene. The promoter region may be shared by ATM and NPAT and that each gene may influence the expression of the other.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nuclear Protein, Ataxia Telangiectasia Locus (NPAT) ELISA Kit Customized Service Offer
  1. "Identification and characterization of a new gene physically linked to the ATM gene."Genome Res. 6:439-447(1996) [PubMed] [Europe PMC] [Abstract]
  2. "A gene transcribed from the bidirectional ATM promoter coding for a serine rich protein: amino acid sequence, structure and expression studies."Hum. Mol. Genet. 5:1785-1791(1996) [PubMed] [Europe PMC] [Abstract]
  3. "The structure and organization of the human NPAT gene."Genomics 42:388-392(1997) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "CAND3: a ubiquitously expressed gene immediately adjacent and in opposite transcriptional orientation to the ATM gene at 11q23.1."Mamm. Genome 8:129-133(1997) [PubMed] [Europe PMC] [Abstract]
  7. "Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry."Genes Dev. 12:456-461(1998) [PubMed] [Europe PMC] [Abstract]
  8. "NPAT links cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription."Genes Dev. 14:2283-2297(2000) [PubMed] [Europe PMC] [Abstract]
  9. "Cell cycle-regulated phosphorylation of p220(NPAT) by cyclin E/Cdk2 in Cajal bodies promotes histone gene transcription."Genes Dev. 14:2298-2313(2000) [PubMed] [Europe PMC] [Abstract]
  10. "Aberrant methylation of the ATM promoter correlates with increased radiosensitivity in a human colorectal tumor cell line."Oncogene 21:3864-3871(2002) [PubMed] [Europe PMC] [Abstract]
  11. "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component."Cell 114:255-266(2003) [PubMed] [Europe PMC] [Abstract]
  12. "NPAT expression is regulated by E2F and is essential for cell cycle progression."Mol. Cell. Biol. 23:2821-2833(2003) [PubMed] [Europe PMC] [Abstract]
  13. "The cyclin E/Cdk2 substrate and Cajal body component p220(NPAT) activates histone transcription through a novel LisH-like domain."Mol. Cell. Biol. 23:3669-3680(2003) [PubMed] [Europe PMC] [Abstract]
  14. "Identification of HiNF-P, a key activator of cell cycle-controlled histone H4 genes at the onset of S phase."Mol. Cell. Biol. 23:8110-8123(2003) [PubMed] [Europe PMC] [Abstract]
  15. "The cyclin E/Cdk2 substrate p220(NPAT) is required for S-phase entry, histone gene expression, and Cajal body maintenance in human somatic cells."Mol. Cell. Biol. 23:8586-8600(2003) [PubMed] [Europe PMC] [Abstract]
  16. "Dynamic interaction of p220(NPAT) and CBP/p300 promotes S-phase entry."Biochem. Biophys. Res. Commun. 325:1509-1516(2004) [PubMed] [Europe PMC] [Abstract]
  17. "DNA damage induces downregulation of histone gene expression through the G1 checkpoint pathway."EMBO J. 23:1133-1143(2004) [PubMed] [Europe PMC] [Abstract]
  18. "Coordinate control and selective expression of the full complement of replication-dependent histone H4 genes in normal and cancer cells."J. Biol. Chem. 280:37400-37407(2005) [PubMed] [Europe PMC] [Abstract]
  19. "HiNF-P directly links the cyclin E/CDK2/p220NPAT pathway to histone H4 gene regulation at the G1/S phase cell cycle transition."Mol. Cell. Biol. 25:6140-6153(2005) [PubMed] [Europe PMC] [Abstract]
  20. "Deficiency of the zinc finger protein ZPR1 causes defects in transcription and cell cycle progression."J. Biol. Chem. 281:40330-40340(2006) [PubMed] [Europe PMC] [Abstract]
  21. "Self-renewal of human embryonic stem cells is supported by a shortened G1 cell cycle phase."J. Cell. Physiol. 209:883-893(2006) [PubMed] [Europe PMC] [Abstract]
  22. "FLASH is required for histone transcription and S-phase progression."Proc. Natl. Acad. Sci. U.S.A. 103:14808-14812(2006) [PubMed] [Europe PMC] [Abstract]
  23. "The HiNF-P/p220NPAT cell cycle signaling pathway controls nonhistone target genes."Cancer Res. 67:10334-10342(2007) [PubMed] [Europe PMC] [Abstract]
  24. "Transcriptional activation of the histone nuclear factor P (HiNF-P) gene by HiNF-P and its cyclin E/CDK2 responsive co-factor p220NPAT defines a novel autoregulatory loop at the G1/S phase transition."Gene 402:94-102(2007) [PubMed] [Europe PMC] [Abstract]
  25. "HiNF-P is a bifunctional regulator of cell cycle controlled histone H4 gene transcription."J. Cell. Biochem. 101:181-191(2007) [PubMed] [Europe PMC] [Abstract]
  26. "Establishment of histone gene regulation and cell cycle checkpoint control in human embryonic stem cells."J. Cell. Physiol. 210:517-526(2007) [PubMed] [Europe PMC] [Abstract]
  27. "Cell cycle dependent phosphorylation and subnuclear organization of the histone gene regulator p220(NPAT) in human embryonic stem cells."J. Cell. Physiol. 213:9-17(2007) [PubMed] [Europe PMC] [Abstract]
  28. "Transcriptional activation of histone genes requires NPAT-dependent recruitment of TRRAP-Tip60 complex to histone promoters during the G1/S phase transition."Mol. Cell. Biol. 28:435-447(2008) [PubMed] [Europe PMC] [Abstract]
  29. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  30. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  31. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]