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Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8)

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Rub1; Neddylin; Ubiquitin-like protein Nedd8

Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8)
CCC P842
HGNC 7732
MGI 97301
PubMed 24821572
RGD 3158
UniProt Q15843
Wiki Rub1
NEDD8 is activated by an E1-like complex, consisting of beta-amyloid precursor protein-binding protein-1 (APPBP1) and UBA3, and then linked to the E2-like enzyme, UBC12. Nedd8 conjugation negatively regulates contractility of the microfilament-rich cell cortex during pronuclear migration and during cytokinesis.
Human NEDD8 and ubiquitin share 60% amino acid sequence identity. By Western blot analysis of human cell line lysates, NEDD8 migrated as a 6-kD protein. Immunofluorescence studies showed that NEDD8 is expressed primarily in the nucleus. As with ubiquitin and sentrin, NEDD8 is conjugated to other cellular proteins after its C-terminal tail is processed. Northern blot analysis detected NEDD8 expression predominantly in heart and skeletal muscle.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Neural Precursor Cell Expressed, Developmentally Down Regulated 8 (NEDD8) ELISA Kit Customized Service Offer
  1. "A new NEDD8-ligating system for cullin-4A."Genes Dev. 12:2263-2268(1998) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 is linked to von Hippel-Lindau tumor suppressor function."Proc. Natl. Acad. Sci. U.S.A. 96:5510-5515(1999) [PubMed] [Europe PMC] [Abstract]
  4. "Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein."J. Biol. Chem. 272:28557-28562(1997) [PubMed] [Europe PMC] [Abstract]
  5. "Cleavage of the C-terminus of NEDD8 by UCH-L3."Biochem. Biophys. Res. Commun. 251:688-692(1998) [PubMed] [Europe PMC] [Abstract]
  6. "Covalent modification of all members of human cullin family proteins by NEDD8."Oncogene 18:6829-6834(1999) [PubMed] [Europe PMC] [Abstract]
  7. "NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression."J. Biol. Chem. 276:20603-20609(2001) [PubMed] [Europe PMC] [Abstract]
  8. "The NEDD8 pathway is essential for SCF(beta -TrCP)-mediated ubiquitination and processing of the NF-kappa B precursor p105."J. Biol. Chem. 277:23253-23259(2002) [PubMed] [Europe PMC] [Abstract]
  9. "Interaction with Nedd8, a ubiquitin-like protein, enhances the transcriptional activity of the aryl hydrocarbon receptor."J. Biol. Chem. 277:44028-44034(2002) [PubMed] [Europe PMC] [Abstract]
  10. "NEDP1, a highly conserved cysteine protease that deneddylates cullins."J. Biol. Chem. 278:25637-25643(2003) [PubMed] [Europe PMC] [Abstract]
  11. "DEN1 is a dual function protease capable of processing the C-terminus of Nedd8 and deconjugating hyper-neddylated CUL1."J. Biol. Chem. 278:28882-28891(2003) [PubMed] [Europe PMC] [Abstract]
  12. "Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity."Cell 118:83-97(2004) [PubMed] [Europe PMC] [Abstract]
  13. "Crystal structure of the human ubiquitin-like protein NEDD8 and interactions with ubiquitin pathway enzymes."J. Biol. Chem. 273:34983-34991(1998) [PubMed] [Europe PMC] [Abstract]
  14. "The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1."Mol. Cell 12:1427-1437(2003) [PubMed] [Europe PMC] [Abstract]
  15. "Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1."J. Mol. Biol. 345:141-151(2005) [PubMed] [Europe PMC] [Abstract]