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HUS1 Like Protein (HUS1)

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HUS1 Checkpoint Homolog

HUS1 Like Protein (HUS1)
CCC P942
HGNC 5309
MGI 1277962
PubMed 23468651
RGD 1591976
UniProt O60921
Wiki HUS1
HUS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. The predicted 281-amino acid human protein shares 30% and 86% identity with S. pombe hus1 and mouse Hus1, respectively.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant HUS1 Like Protein (HUS1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to HUS1 Like Protein (HUS1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to HUS1 Like Protein (HUS1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for HUS1 Like Protein (HUS1) CLIA Kit Customized Service Offer
n/a ELISA Kit for HUS1 Like Protein (HUS1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant HUS1 Like Protein (HUS1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to HUS1 Like Protein (HUS1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to HUS1 Like Protein (HUS1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for HUS1 Like Protein (HUS1) CLIA Kit Customized Service Offer
n/a ELISA Kit for HUS1 Like Protein (HUS1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant HUS1 Like Protein (HUS1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to HUS1 Like Protein (HUS1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to HUS1 Like Protein (HUS1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for HUS1 Like Protein (HUS1) CLIA Kit Customized Service Offer
n/a ELISA Kit for HUS1 Like Protein (HUS1) ELISA Kit Customized Service Offer
  1. "Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage."EMBO J. 17:2055-2066(1998) [PubMed] [Europe PMC] [Abstract]
  2. "cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster."Genomics 54:424-436(1998) [PubMed] [Europe PMC] [Abstract]
  3. "Physical interaction among human checkpoint control proteins HUS1p, RAD1p, and RAD9p, and implications for the regulation of cell cycle progression."Genomics 65:24-33(2000) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence of human chromosome 7." Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1."Mol. Biol. Cell 10:1985-1995(1999) [PubMed] [Europe PMC] [Abstract]
  8. "HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins."J. Biol. Chem. 275:27909-27916(2000) [PubMed] [Europe PMC] [Abstract]
  9. "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9."J. Biol. Chem. 275:29767-29771(2000) [PubMed] [Europe PMC] [Abstract]
  10. "PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition."Oncogene 19:5291-5297(2000) [PubMed] [Europe PMC] [Abstract]
  11. "The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9."Biochem. Biophys. Res. Commun. 287:932-940(2001) [PubMed] [Europe PMC] [Abstract]
  12. "Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue."Cancer Res. 63:5291-5298(2003) [PubMed] [Europe PMC] [Abstract]
  13. "Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene."Genomics 82:644-651(2003) [PubMed] [Europe PMC] [Abstract]
  14. "Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro."Proc. Natl. Acad. Sci. U.S.A. 100:1633-1638(2003) [PubMed] [Europe PMC] [Abstract]
  15. "The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair."Nucleic Acids Res. 32:3316-3324(2004) [PubMed] [Europe PMC] [Abstract]
  16. "The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1."Proc. Natl. Acad. Sci. U.S.A. 101:16762-16767(2004) [PubMed] [Europe PMC] [Abstract]
  17. "The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery."Biochem. J. 389:13-17(2005) [PubMed] [Europe PMC] [Abstract]
  18. "The two DNA clamps Rad9/Rad1/Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity."J. Mol. Biol. 353:980-989(2005) [PubMed] [Europe PMC] [Abstract]
  19. "Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells."Oncogene 24:4728-4735(2005) [PubMed] [Europe PMC] [Abstract]
  20. "A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling."Science 332:1313-1317(2011) [PubMed] [Europe PMC] [Abstract]