Dihydrodiol Dehydrogenase 2 (DDH2)
[Edit]AKR1C2; DD; BABP; DD2; HAKRD; MCDR2; Aldo-Keto Reductase Family 1,Member C2; Bile Acid Binding Protein; 3-Alpha Hydroxysteroid Dehydrogenase Type III
Dihydrodiol dehydrogenase (DD; EC 1.3.1.20), a member of the aldo-oxo reductase (AKR) superfamily, catalyzes the NADP-linked oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols. DDH2 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
Organism species: Homo sapiens (Human)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPQ200Hu01 | Recombinant Dihydrodiol Dehydrogenase 2 (DDH2) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAQ200Hu01 | Polyclonal Antibody to Dihydrodiol Dehydrogenase 2 (DDH2) | WB; IHC; ICC; IP. |
MAQ200Hu21 | Monoclonal Antibody to Dihydrodiol Dehydrogenase 2 (DDH2) | WB; IHC; ICC; IP. | |
Assay Kits | SEQ200Hu | ELISA Kit for Dihydrodiol Dehydrogenase 2 (DDH2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Mus musculus (Mouse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | n/a | Recombinant Dihydrodiol Dehydrogenase 2 (DDH2) | Recombinant Protein Customized Service Offer |
Antibodies | n/a | Monoclonal Antibody to Dihydrodiol Dehydrogenase 2 (DDH2) | Monoclonal Antibody Customized Service Offer |
n/a | Polyclonal Antibody to Dihydrodiol Dehydrogenase 2 (DDH2) | Polyclonal Antibody Customized Service Offer | |
Assay Kits | n/a | CLIA Kit for Dihydrodiol Dehydrogenase 2 (DDH2) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Dihydrodiol Dehydrogenase 2 (DDH2) | ELISA Kit Customized Service Offer |
Organism species: Rattus norvegicus (Rat)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | n/a | Recombinant Dihydrodiol Dehydrogenase 2 (DDH2) | Recombinant Protein Customized Service Offer |
Antibodies | n/a | Monoclonal Antibody to Dihydrodiol Dehydrogenase 2 (DDH2) | Monoclonal Antibody Customized Service Offer |
n/a | Polyclonal Antibody to Dihydrodiol Dehydrogenase 2 (DDH2) | Polyclonal Antibody Customized Service Offer | |
Assay Kits | n/a | CLIA Kit for Dihydrodiol Dehydrogenase 2 (DDH2) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Dihydrodiol Dehydrogenase 2 (DDH2) | ELISA Kit Customized Service Offer |
- "Molecular cloning of multiple cDNAs encoding human enzymes structurally related to 3 alpha-hydroxysteroid dehydrogenase."J. Steroid Biochem. Mol. Biol. 46:673-679(1993) [PubMed] [Europe PMC] [Abstract]
- "cDNA and deduced amino acid sequences of a human colon dihydrodiol dehydrogenase."Biochim. Biophys. Acta 1186:129-132(1994) [PubMed] [Europe PMC] [Abstract]
- "Structure of a gene coding for human dihydrodiol dehydrogenase/bile acid-binding protein."Gene 149:357-361(1994) [PubMed] [Europe PMC] [Abstract]
- "Molecular cloning of human type 3 3 alpha-hydroxysteroid dehydrogenase that differs from 20 alpha-hydroxysteroid dehydrogenase by seven amino acids."Biochem. Biophys. Res. Commun. 228:474-479(1996) [PubMed] [Europe PMC] [Abstract]
- "Sequence of the cDNA of a human dihydrodiol dehydrogenase isoform (AKR1C2) and tissue distribution of its mRNA."Biochem. J. 334:399-405(1998) [PubMed] [Europe PMC] [Abstract]
- "Close kinship of human 20alpha-hydroxysteroid dehydrogenase gene with three aldo-keto reductase genes."Genes Cells 5:111-125(2000) [PubMed] [Europe PMC] [Abstract]
- "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
- "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "Relationship of human liver dihydrodiol dehydrogenases to hepatic bile-acid-binding protein and an oxidoreductase of human colon cells."Biochem. J. 313:373-376(1996) [PubMed] [Europe PMC] [Abstract]
- "cDNA cloning and expression of the human hepatic bile acid-binding protein. A member of the monomeric reductase gene family."J. Biol. Chem. 268:10448-10457(1993) [PubMed] [Europe PMC] [Abstract]
- "Why boys will be boys: two pathways of fetal testicular androgen biosynthesis are needed for male sexual differentiation."Am. J. Hum. Genet. 89:201-218(2011) [PubMed] [Europe PMC] [Abstract]
- "Crystal structure of human type III 3alpha-hydroxysteroid dehydrogenase/bile acid binding protein complexed with NADP(+) and ursodeoxycholate."Biochemistry 40:10161-10168(2001) [PubMed] [Europe PMC] [Abstract]
- "Structure of the human 3alpha-hydroxysteroid dehydrogenase type 3 in complex with testosterone and NADP at 1.25-A resolution."J. Biol. Chem. 276:42091-42098(2001) [PubMed] [Europe PMC] [Abstract]
- "Comparison of crystal structures of human type 3 3alpha-hydroxysteroid dehydrogenase reveals an 'induced-fit' mechanism and a conserved basic motif involved in the binding of androgen."Protein Sci. 14:1485-1497(2005) [PubMed] [Europe PMC] [Abstract]
- "Crystal structures of mouse 17alpha-hydroxysteroid dehydrogenase (apoenzyme and enzyme-NADP(H) binary complex): identification of molecular determinants responsible for the unique 17alpha-reductive activity of this enzyme."J. Mol. Biol. 364:747-763(2006) [PubMed] [Europe PMC] [Abstract]
- "Mouse 17alpha-hydroxysteroid dehydrogenase (AKR1C21) binds steroids differently from other aldo-keto reductases: identification and characterization of amino acid residues critical for substrate binding."J. Mol. Biol. 369:525-540(2007) [PubMed] [Europe PMC] [Abstract]