Adaptor Related Protein Complex 5 Zeta 1 (AP5z1)

KIAA0415 exists in a core protein complex containing SPG11, SPG15 (ZFYVE26), C20ORF29, and DKFZp761E198. Knockdown of KIAA0415, C20ORF29, or SPG15 in HeLa cells reduced homologous recombination in a test substrate, whereas knockdown of SPG11 or DKFZp761E198 had no effect. Knockdown of KIAA0415 also reduced homologous recombination in U2OS human osteosarcoma cells.The deduced 634-amino acid protein shares weak similarity with cyclin A1 (CCNA1). RT-PCR detected variable KIAA0415 expression in all tissues examined. In vitro-translated KIAA0415 had an apparent molecular mass of 68 kD by SDS-PAGE.KIAA0415 has a putative C-terminal helicase domain characteristic of superfamily 2 (SF2)-type helicases.