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Mitochondrial Poly A Polymerase (MTPAP)

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PAPD1; mtPAP; TUTase1; Polynucleotide adenylyltransferase; PAP Associated Domain Containing 1; Terminal uridylyltransferase 1; Poly(A) RNA polymerase, mitochondrial

Mitochondrial Poly A Polymerase (MTPAP)
CCC R433
HGNC 25532
MGI 1914690
RGD 1310900
UniProt Q9NVV4
Mammalian mitochondrial (mt) mRNAs have short poly(A) tails at their 3' termini that are post-transcriptionally synthesized by mt poly(A) polymerase (PAP). Partial inactivation of hmtPAP by RNA interference using small interfering RNA in HeLa cells resulted in shortened poly(A) tails and decreased steady state levels of some mt mRNAs as well as their translational products. Moreover, knocking down hmtPAP generated markedly defective mt membrane potentials and reduced oxygen consumption. In contrast, knocking down hPNPase showed significantly extended poly(A) tails of mt mRNAs. These results demonstrate that the poly(A) length of human mt mRNAs is controlled by polyadenylation by hmtPAP and deadenylation by hPNPase, and polyadenylation is required for the stability of mt mRNAs.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mitochondrial Poly A Polymerase (MTPAP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mitochondrial Poly A Polymerase (MTPAP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mitochondrial Poly A Polymerase (MTPAP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mitochondrial Poly A Polymerase (MTPAP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mitochondrial Poly A Polymerase (MTPAP) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mitochondrial Poly A Polymerase (MTPAP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mitochondrial Poly A Polymerase (MTPAP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mitochondrial Poly A Polymerase (MTPAP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mitochondrial Poly A Polymerase (MTPAP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mitochondrial Poly A Polymerase (MTPAP) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mitochondrial Poly A Polymerase (MTPAP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mitochondrial Poly A Polymerase (MTPAP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mitochondrial Poly A Polymerase (MTPAP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mitochondrial Poly A Polymerase (MTPAP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mitochondrial Poly A Polymerase (MTPAP) ELISA Kit Customized Service Offer
  1. "Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase."Nucleic Acids Res. 32:6001-6014(2004) [PubMed] [Europe PMC] [Abstract]
  2. "Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase."J. Biol. Chem. 280:19721-19727(2005) [PubMed] [Europe PMC] [Abstract]
  3. "NovelFam3000 -- uncharacterized human protein domains conserved across model organisms."BMC Genomics 7:48-48(2006) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  6. "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'."Genes Dev. 22:50-65(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  11. "Structural basis for dimerization and activity of human PAPD1, a noncanonical poly(A) polymerase."Mol. Cell 41:311-320(2011) [PubMed] [Europe PMC] [Abstract]
  12. "Defective mitochondrial mRNA maturation is associated with spastic ataxia."Am. J. Hum. Genet. 87:655-660(2010) [PubMed] [Europe PMC] [Abstract]