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Ubiquitin Conjugating Enzyme E2W (UBE2W)

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UBC16; N-terminus-conjugating E2; Ubiquitin carrier protein W; Ubiquitin-conjugating enzyme 16; Ubiquitin-protein ligase W

Ubiquitin Conjugating Enzyme E2W (UBE2W)
CCC R453
HGNC 25616
MGI 1914049
PubMed 21229326
UniProt Q96B02
UBE2W, a newly described member of E2 family, was formerly reported probably involving in phototransduction or retinal degeneration in Drosophila. Murine UBE2W harbors a typical UBC domain and is highly conserved in different vertebrate homologues. GST-tagged UBE2W was expressed in E. coli BL21 (DE3) and purified with GST affinity chromatography. The activity and specificity were confirmed by immunoblotting of transiently expressed myc-UBE2W fusion protein.
Wide expression of UBE2W was found in brain, muscle, heart, lung, liver, spleen, kidney and testis of mouse with the generated antibody, indicating the functional importance of this novel protein. Furthermore, the UBE2W highly expression was confined to the adult testis and was developmental stage-specific.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ubiquitin Conjugating Enzyme E2W (UBE2W) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2W (UBE2W) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2W (UBE2W) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ubiquitin Conjugating Enzyme E2W (UBE2W) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ubiquitin Conjugating Enzyme E2W (UBE2W) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ubiquitin Conjugating Enzyme E2W (UBE2W) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2W (UBE2W) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2W (UBE2W) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ubiquitin Conjugating Enzyme E2W (UBE2W) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ubiquitin Conjugating Enzyme E2W (UBE2W) ELISA Kit Customized Service Offer
  1. "Cloning, characterization and subcellular localization of a gene encoding a human Ubiquitin-conjugating enzyme (E2) homologous to the Arabidopsis thaliana UBC-16 gene product."Front. Biosci. 11:1500-1507(2006) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "DNA sequence and analysis of human chromosome 8." Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Mechanistic insight into site-restricted monoubiquitination of FANCD2 by Ube2t, FANCL, and FANCI."Mol. Cell 32:767-777(2008) [PubMed] [Europe PMC] [Abstract]
  6. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
  7. "UBE2W interacts with FANCL and regulates the monoubiquitination of Fanconi anemia protein FANCD2."Mol. Cells 31:113-122(2011) [PubMed] [Europe PMC] [Abstract]
  8. "Ube2W conjugates ubiquitin to alpha-amino groups of protein N-termini."Biochem. J. 453:137-145(2013) [PubMed] [Europe PMC] [Abstract]
  9. "The ubiquitin-conjugating enzyme (E2) Ube2w ubiquitinates the N terminus of substrates."J. Biol. Chem. 288:18784-18788(2013) [PubMed] [Europe PMC] [Abstract]
  10. "A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen."Mol. Cell. Proteomics 11:329-341(2012) [PubMed] [Europe PMC] [Abstract]