SLAIN Motif Family, Member 1 (SLAIN1)

SLAIN1 contain 629 and 640 amino acids, respectively, and share 86.3% identity. SLAIN1 has an N-terminal proline-rich domain and a serine-rich domain. Northern blot analysis detected high expression of Slain1 in mouse thymus and lower expression in brain, testis, and lung. Semiquantitative RT-PCR detected Slain1 expression in mouse bone marrow, brain, heart, kidney, lung, testis, and thymus, but not in liver. Slain1 was highly expressed at the stem cell and epiblast stages of mouse embryonic stem cell development. Human stem cells also showed highest SLAIN1 levels prior to differentiation. In situ hybridization of the developing mouse embryo revealed Slain1 expression in the neural tube and optic vesicles at day 8.5, and then at sites of imminent neural tube closure in the midbrain, hindbrain, and tailbud and in the dorsal aspects of somites.