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Transmembrane Protein 74 (TMEM74)

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NET36

Transmembrane Protein 74 (TMEM74)
CCC R617
HGNC 26409
MGI 2443417
PubMed 18294959
RGD 1566279
UniProt Q96NL1
A novel autophagy-related molecule, Transmembrane protein 74 (TMEM74). Its transcript was identified by Northern blot and the open reading frame was found to encode 393 amino acids, which shared very little identity with other genetic products. Subcellular localization analysis showed TMEM74 localized to the lysosome and autophagosome. Overexpression of TMEM74 in HeLa cells resulted in autophagic vacuolization, increased the dotted distribution of MDC and GFP-LC3, and endogenous LC3-II levels. Wortmannin, an autophagy inhibitor, partially attenuated these effects. Moreover, knockdown of TMEM74 by small interference RNA abolished the autophagic characteristics induced by starvation. These findings demonstrate that TMEM74 may be involved in promoting functional autophagy during cell starvation and other stress conditions.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Transmembrane Protein 74 (TMEM74) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Transmembrane Protein 74 (TMEM74) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Transmembrane Protein 74 (TMEM74) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Transmembrane Protein 74 (TMEM74) CLIA Kit Customized Service Offer
n/a ELISA Kit for Transmembrane Protein 74 (TMEM74) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Transmembrane Protein 74 (TMEM74) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Transmembrane Protein 74 (TMEM74) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Transmembrane Protein 74 (TMEM74) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Transmembrane Protein 74 (TMEM74) CLIA Kit Customized Service Offer
n/a ELISA Kit for Transmembrane Protein 74 (TMEM74) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Transmembrane Protein 74 (TMEM74) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Transmembrane Protein 74 (TMEM74) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Transmembrane Protein 74 (TMEM74) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Transmembrane Protein 74 (TMEM74) CLIA Kit Customized Service Offer
n/a ELISA Kit for Transmembrane Protein 74 (TMEM74) ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "TMEM74, a lysosome and autophagosome protein, regulates autophagy."Biochem. Biophys. Res. Commun. 369:622-629(2008) [PubMed] [Europe PMC] [Abstract]
  4. "High-throughput functional screening for autophagy-related genes and identification of TM9SF1 as an autophagosome-inducing gene."Autophagy 5:52-60(2009) [PubMed] [Europe PMC] [Abstract]