Enolase 4 (ENO4)

ENO4, Belongs to the enolase family. Although it is highly related to enolase enzyme family, lacks the conserved Glu active site at position 353, which is replaced by a Gln residue. It is therefore unsure whether it has enolase activity.

Enolase, also known as phosphopyruvate dehydratase, is a metalloenzyme responsible for the catalysis of the conversion of 2-phosphoglycerate (2-PG) to phosphoenolpyruvate (PEP), the ninth and penultimate step of glycolysis. Enolase can also catalyze the reverse reaction, depending on environmental concentrations of substrates. The optimum pH for this enzyme is 6.5. Enolase is present in all tissues and organisms capable of glycolysis or fermentation.Enolase has a molecular weight of 82,000-100,000 Daltons depending on the isoform.