Melanoma Associated Antigen 1 Like Protein 1 (MUM1L1)

MUM1L1 belongs to the MUM1 family. Contains 1 PWWP domain. Microarray expression analysis of thyroid cancer cell lines has implicated a member of the melanoma-associated family of cancer–testis antigens in thyroid cancer development and progression. A tissue microarray (TMA) of samples from 375 patients with thyroid cancer was analyzed with immunohistochemistry to localize MAGE. Western blotting of fractionated proteins from MAGE-transfected cells was used to confirm intracellular localization of proteins. Automated analysis of TMA samples was evaluated and subjected to statistical analysis. MAGE immunoreactivity was identified in nuclear and cytoplasmic compartments of normal and malignant tissues. The use of TMA analyses identifies IHC techniques that are translatable to the clinical setting for prognostic assessment of patients with thyroid cancer.