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Active Activin A (ACVA)

ACV-A; Activin Beta A Beta A Homodimer

ACTIVITY TEST

Activin A (ACVA) is a member of the TGF-β superfamily of cytokines and involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis. To test the effect of ACVA on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 3,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant human ACVA. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2 hours at 37℃. Proliferation of A549 cells after incubation with ACVA for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human ACVA for 48h. The result was shown in Figure 2. It was obvious that ACVA significantly inhibit cell viability of A549 cells. The ED50 is 5.09 μg/mL.

Figure 2. Inhibition of A549 cells proliferation after stimulated with ACVA.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
13 Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte Dysfunction Ahajournals: Source
J. Pharmacogn Article: Hksobf_2015_V46N2_167
United States Patent Application Methods for Diagnosis and Treatment of Concussion or Brain Injury 2016:0103140
BMC Cancer. Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. pubmed:27835986
Basic Clin Pharmacol Toxicol. Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. pubmed:28032440
Basic & Clinical Pharmacology & Toxicology Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling doi:10.1111
Journal of Immunology MicroRNA-27b Modulates Inflammatory Response and Apoptosis during Mycobacterium tuberculosis Infection Pubmed:29661829
Journal of Vascular Surgery Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissection Pubmed:29685513
Acta Biochimica et Biophysica Sinica Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis 10.1093:abbs
Poultry Science The effect of raw and fermented rapeseed cake on the metabolic parameters, immune status, and intestinal morphology of turkeys Pubmed:29917099
Food Bioscience Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity 10.1016:j.fbio.2018.06.002
Current Analytical Chemistry Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A 10.2174:2211556006666170525161225
Journal of Veterinary Internal Medicine FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB :
Universal Journal of Pharmaceutical Research SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY-YEMEN 10.22270:ujpr.v3i2.138
Involvement of RBP4 in diabetic atherosclerosis and the role of vitamin D intervention :
Clin Oral Investig.  Gingival crevicular fluid levels of human beta-defensin-1 in type 2 diabetes mellitus and periodontitis Pubmed:29713888
Myocardial fibrosis in hypertrophic cardiomyopathy :
TELLING TRANSVERSAL COMPETENCES… TO BE PROFESSIONALLY PROMOTED :
Protein biomarker panels for detecting colorectal cancer and advanced adenoma :
Endothelotropic activity of 4-hydroxy-3, 5-di-tret-butylcinnamic acid in the conditions of experimental cerebral ischemia :
Silencing of the CSNK2β gene by siRNA inhibits invasiveness and growth of MDA-MB-231 cells 10.1101:311092
Duchenne Muscular Dystrophy Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular Dystrophy Pubmed:29067667
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Journal of Geriatric Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors Pubmed: 30952517
clinica chimica acta The performances of serum activins and follistatin in the diagnosis of ectopic pregnancy: A prospective case-control study Pubmed: 31647905
hindawi The Association between the Activin A Serum Level and Carotid Intima-Media Thickness in Chronic Kidney Disease Patients
Pathology & Oncology Research Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status 34867090
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA001Hu01 Recombinant Activin A (ACVA) Positive Control; Immunogen; SDS-PAGE; WB.
APA001Hu01 Active Activin A (ACVA) Cell culture; Activity Assays.
PAA001Hu01 Polyclonal Antibody to Activin A (ACVA) WB; ICC; IHC-P; IHC-F; ELISA.
SEA001Hu ELISA Kit for Activin A (ACVA) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA001Hu ELISA Kit for Activin A (ACVA) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA001Hu CLIA Kit for Activin A (ACVA) Chemiluminescent immunoassay for Antigen Detection.
LMA001Hu Multiplex Assay Kit for Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.