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Active Angiogenin (ANG)

RNASE5; Ribonuclease,RNase A Family 5

ACTIVITY TEST

Angiogenin (ANG) was initially purified from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29 based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane. A number of other tumor, as well as normal, cell lines can also secrete Angiogenin. In addition, Angiogenin is present in normal human plasma at levels as high as 60-120 ng/mL. Surprisingly, Angiogenin has been found to be a member of the ribonuclease superfamily with approximately 35% sequence similarity at the amino acid level with pancreatic RNase. Angiogenin exhibits ribonucleolytic activity that is distinctly different than that of pancreatic RNase A. The ribonucleolytic activity of Angiogenin toward most RNase A substrates is much lower than that of RNase A. Nevertheless, the ribonucleolytic activity of Angiogenin is essential to its angiogenic activity since inhibition of the Angiogenin RNase activity will also abolish angiogenesis activity. The activity of recombinant mouse ANG was measured by its ribonucleolytic activity toward RNA. The result was shown in figure 1, and the rmANG produces a delta Abs260/μg >1.0 in two hours.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Inflammopharmacology Evening primrose oil and celecoxib inhibited pathological angiogenesis, inflammation, and oxidative stress in adjuvant-induced arthritis: novel role of angiopoietin-1 Pubmed: 24664592
Cancer Cell Angiogenin/Ribonuclease 5 is an EGFR ligand and a serum biomarker for erlotinib sensitivity in pancreatic cancer Pubmed:29606349
Journal of Immunotherapy The VE-PTP Inhibitor AKB-9778 Improves Antitumor Activity and Diminishes the Toxicity of Interleukin 2 (IL-2) Administration Pubmed: 31348125
Clinical Hemorheology and Microcirculation Nonselective alpha-/beta-AR antagonists can inhibit pericyte proliferation, migration, and secretion in vitro Pubmed: 32224529
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APA007Mu02 Active Angiogenin (ANG) Cell culture; Activity Assays.
RPA007Mu01 Recombinant Angiogenin (ANG) Positive Control; Immunogen; SDS-PAGE; WB.
RPA007Mu02 Recombinant Angiogenin (ANG) Positive Control; Immunogen; SDS-PAGE; WB.
PAA007Mu01 Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
PAA007Mu02 Polyclonal Antibody to Angiogenin (ANG) WB; IHC; ICC; IP.
SEA007Mu ELISA Kit for Angiogenin (ANG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA007Mu Multiplex Assay Kit for Angiogenin (ANG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.