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Active Bone Morphogenetic Protein 4 (BMP4)

BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B

ACTIVITY TEST

BMP4 (Bone morphogenetic protein 4) is a member of the bone morphogenetic protein family, which is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. To test the effect of BMP4 on cell apoptosis, HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant rat BMP4 diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Apoptosis of HepG2 cells after incubation with BMP4 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant rat BMP4 for 72h. The result was shown in Figure 2. It was obvious that BMP4 significantly decreased cell viability of HepG2 cells. The ED50 of recombinant rat BMP4 is 18.5 ug/ml.

Figure 2. Inhibition of HepG2 cells proliferation after stimulated with recombinant rat BMP4

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Journal of Cellular Biochemistry Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway PubMed: 26681207
Domestic Animal Endocrinology Expression analysis of bone morphogenetic protein 4 between fat and lean birds in adipose tissue and serum Pubmed:26945137
FEBS Open Bio Bone morphogenetic protein 4 (BMP 4) regulates immortalized chicken preadipocyte proliferation by promoting G1/S cell cycle progression Pubmed: 30972973
Arch Gynecol Obstet The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese … 34417839
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA014Ra01 Recombinant Bone Morphogenetic Protein 4 (BMP4) Positive Control; Immunogen; SDS-PAGE; WB.
RPA014Ra02 Recombinant Bone Morphogenetic Protein 4 (BMP4) Positive Control; Immunogen; SDS-PAGE; WB.
APA014Ra02 Active Bone Morphogenetic Protein 4 (BMP4) Cell culture; Activity Assays.
PAA014Ra01 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4) WB; IHC; ICC; IP.
PAA014Ra02 Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4) WB; IHC; ICC; IP.
LAA014Ra71 Biotin-Linked Polyclonal Antibody to Bone Morphogenetic Protein 4 (BMP4) WB; IHC; ICC.
SCA014Ra CLIA Kit for Bone Morphogenetic Protein 4 (BMP4) Chemiluminescent immunoassay for Antigen Detection.