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Active E-cadherin

CDHE; CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule

ACTIVITY TEST

E-Cadherin, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Human E-Cadherin shares 81% amino acid sequence identity with the rat and mouse proteins. It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. As E-cadherin has the function of cell adhesion, we measure the activity of recombinant human E-cadherin by the ability of the immobilized protein to support the adhesion of MCF7 human breast cancer cells. When 5 x 104 cells/well are added to different concentrations of recombinat human E-cadherin coated plates, cells will adhere after 2 hour incubation at 37 ℃. The adhesion of MCF7 after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.23 ug/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
J Periodontal Res. Novel method for proliferation of oral keratinocyte stem cells Pubmed: 24329011
The Journal of Gene Medicine Modulation of the Dual‐Faced Effects of miR‐141 with Chitosan/miR‐141 Nanoplexes in Breast Cancer Cells Pubmed: 31389101
Cell Adhesion & Migration BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin Pubmed: 31957537
DOKLADY BIOCHEMISTRY AND BIOPHYSICS Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … Pubmed: 32342301
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …
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PAA017Hu02 Polyclonal Antibody to E-cadherin WB; IHC; ICC; IP.
LAA017Hu71 Biotin-Linked Polyclonal Antibody to E-cadherin WB; IHC; ICC.
LAA017Hu81 FITC-Linked Polyclonal Antibody to E-cadherin WB; IHC; ICC; IF.
MAA017Hu23 Monoclonal Antibody to E-cadherin WB; IHC; ICC; IP.
MAA017Hu22 Monoclonal Antibody to E-cadherin WB; IHC; ICC; IP.
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