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Active Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF)

PK1; EGVEGF; PRK1, PROK1; Prokineticin 1; Black Mamba Toxin-Related Protein; Mambakine

ACTIVITY TEST

Figure 1. Cell proliferation of ECV-304 cells after stimulated with EG-VEGF.
Endothelial gland-derived VEGF (EG-VEGF) is an angiogenic protein that is structurally unrelated to VEGF. It is expressed in steroidogenic tissues such as adrenal gland, ovary, testis, and placenta. Like VEGF it can induce fenestrae in endothelial cells. To test the effect of EG-VEGF on cell proliferation of ECV-304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of EG-VEGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37℃.
(A) Unstimulated ECV-304 cells cultured in 1640 for 96h;
(B) ECV-304 cells cultured in 1640, stimulated with 10ng/mL VEGF121 for 96h.

Figure. Cell proliferation of ECV-304 cells after stimulated with EG-VEGF

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA024Hu01 Recombinant Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Positive Control; Immunogen; SDS-PAGE; WB.
APA024Hu01 Active Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Cell culture; Activity Assays.
PAA024Hu01 Polyclonal Antibody to Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) WB; IHC; ICC; IP.
MAA024Hu22 Monoclonal Antibody to Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) WB; IHC; ICC; IP.
SEA024Hu ELISA Kit for Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA024Hu CLIA Kit for Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Chemiluminescent immunoassay for Antigen Detection.
LMA024Hu Multiplex Assay Kit for Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.