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Active Fibroblast Growth Factor 1, Acidic (FGF1)

ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta

ACTIVITY TEST

(FGF1) Fibroblast growth factor 1 belongs to the fibroblast growth factor (FGF) family. FGF1 plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 is thought to stimulate the proliferation of 3T3 fibroblasts. Thus, a cell proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF1 using 3T3 fibroblasts. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of 3T3 cells after incubation with FGF1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF1 for 48h. The result was shown in Figure 2. It was obvious that FGF1 significantly increased cell viability of 3T3 cells.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Tissue Engineering Part A Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat Pubmed:24814095
24 Fibroblast growth factor 1 ameliorates diabetic nephropathy by an anti-inflammatory mechanism. pubmed:28750927
Endocrine Connections Characterization of fibroblast growth factor 1 in obese children and adolescents Pubmed:29991637
Molecular Medicine Reports Long non‑coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR‑590‑3p under hypoxic conditions Pubmed: 32323787
Annals of Agricultural and Environmental Medicine Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis 34969228
World J Hepatol Leukocyte cell-derived chemotaxin-2 and fibroblast growth factor 21 in alcohol-induced liver cirrhosis Pubmed:35070009
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA032Mu02 Recombinant Fibroblast Growth Factor 1, Acidic (FGF1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA032Mu01 Recombinant Fibroblast Growth Factor 1, Acidic (FGF1) Positive Control; Immunogen; SDS-PAGE; WB.
APA032Mu01 Active Fibroblast Growth Factor 1, Acidic (FGF1) Cell culture; Activity Assays.
PAA032Mu02 Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1) WB; IHC; ICC; IP.
PAA032Mu01 Polyclonal Antibody to Fibroblast Growth Factor 1, Acidic (FGF1) WB; IHC; ICC; IP.
MEA032Mu Mini Samples ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA032Mu ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA032Mu CLIA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) Chemiluminescent immunoassay for Antigen Detection.
LMA032Mu Multiplex Assay Kit for Fibroblast Growth Factor 1, Acidic (FGF1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.