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Active Hepatocyte Growth Factor (HGF)
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
- Product No.APA047Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point9.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 308
US$ 770
US$ 1540
US$ 4620
US$ 11550
For more details, please contact local distributors!
ACTIVITY TEST
Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells and T cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing. Besides, Matrix Metalloproteinase 9 (MMP9) has been identified as an interactor of HGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HGF and recombinant mouse MMP9. Briefly, HGF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to MMP9 -coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti- HGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human HGF and recombinant mouse MMP9 was shown in Figure 1, the EC50 for this effect is 0.35 ug/mL.
USAGE
Reconstitute in ddH2O to a concentration less than or equal to 0.1mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Gene Therapy and Molecular Biology | Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heart LWW: 01000 |
| Journal of Translational Medicine | Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma BioMed: 14795876 |
| Digestive Diseases and Sciences | Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure SpringerLink: k22823l138x2344x |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children PubMed: PMC3694825 |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) PubMed: PMC3762604 |
| BMC cell biology | BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause PubMed: PMC3640998 |
| Cell Journal (Yakhteh) | Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis PubMed: PMC3584430 |
| Gastroenterol Res Pract | Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis PubMed: 26448742 |
| Tumor Biology | Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151 |
| Cytotherapy | Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury PubMed: 25863963 |
| Biomedicine & Pharmacotherapy | Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats Pubmed:27261582 |
| Can J Physiol Pharmacol. | Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis Pubmed:27186801 |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064 |
| Changes in HGF levels after a progressive test before and after Tabata HIIT program profile:Pawel_Wolanski3 | |
| Stem Cell Research & Therapy | Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats 10.1186/s13287-017-0739-3 |
| Oncology Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c‑Met/PI3K/AKT in vitro 10.3892/ol.2017.7301 |
| Stem Cell Research & Therapy | Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure. pubmed:28679425 |
| Nature Communications | Pulmonary pericytes regulate lung morphogenesis Pubmed:29934496 |
| Experimental And Molecular Medicine | Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke … Pubmed:29650950 |
| Oncology Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro Pubmed:29375714 |
| Bulletin of Experimental Biology and Medicine | Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats Pubmed:29796817 |
| Cell Biology International | Inherent control of hepatocyte proliferationInherent control of hepatocyte proliferation after subtotal liver resection Pubmed: 31297922 |
| Journal of stem cells & regenerative medicine | What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate? Pubmed: 31239605 |
| Open Journal of Veterinary Medicine | Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injury Pubmed: 32821665 |
| Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care 33469783 |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377 |