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Active Insulin Like Growth Factor 1 (IGF1)

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

ACTIVITY TEST

Insulin-like growth factor I (IGF1), is a hormone similar in molecular structure to insulin but have a much higher growth-promoting activity, it belongs to a family of proteins involved in mediating growth and development. It is reported that IGF1 induces the proliferation, migration, differentiation of a large types of cells including the MCF-7 breast cancer cell line. To test the effect of growth factors on proliferation, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of IGF-1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.
Cell proliferation of MCF-7 cells after incubation with IGF1 for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of IGF1 was shown in Figure2. It was obvious that IGF1 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is typically 8.66~17.19 ng/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
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Journal of Neuroendocrinology Interleukin-6 Regulates the Expression of Hypothalamic Neuropeptides Involved in Body Weight in a Gender-Dependent Way Wiley: source
International Journal of Molecular Sciences Insulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in Rats Mdpi: 8562
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Pesquisa Veterinária Brasileira Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian follicles Scielo: Source
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source
Theriogenology Role of insulin-like growth factor-I and follicular fluid from ovarian follicles with different diameters on porcine oocyte maturation and fertilization in vitro ScienceDirect: S0093691X13001659
Biosensors and Bioelectronics Electrochemical immunosensor for the determination of insulin-like growth factor-1 using electrodes modified with carbon nanotubes–poly(pyrrole propionic acid) hybrids ScienceDirect: S0956566313005629
Electromagnetic Biology and Medicine The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source
Nutrition and Cancer A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers Tandfonline: Source
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International Journal of Endocrinology Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal Muscle Cepebr:Source
J Neurol Sci. The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. Pubmed:24629477
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Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831
Int J Clin Exp Med. The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell PubMed: 26628972
Animal and Biomedical Sciences The use of herb mix and lucerne to increase growth rates of dairy heifers System: Files
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Tumor Biology Hypoxia-induced secretion of IL-12 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151
Forage Res RELATIONSHIP OF RESIDUAL FEED INTAKE WITH BLOOD METABOLITES AND HORMONES IN SAHIWAL FEMALE CALVES Wp-Content: Uploads
S Caracteriza Teses: Disponiveis
Photochemistry AND Photobiology Melatonin Mediates Monochromatic Light‐induced Insulin‐like Growth Factor 1 Secretion of Chick Liver: Involvement of Membrane Receptors Pubmed:27128575
Pediatric Pulmonology Protective effect of vitamin D against hyperoxia‐induced lung injury in newborn rats Pubmed:27291304
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Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87
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PLoS One. Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training. pubmed:27880816
international journal of molecular sciences Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. pubmed:28216608
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Molecular Medicine Reports Mesenchymal stem cells promote endothelial progenitor cell proliferation by secreting insulin‑like growth factor‑1 pubmed:28627605
Neurochem Res Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsy pubmed:28078614
Biological Trace Element Research Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007
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International Journal of Chemical Studies Studies on quality attributes of skimmed colostrum powder P-ISSN: 2349–8528
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PLOS one A metabolite of leucine (β-hydroxy-β-methylbutyrate) given to sows during pregnancy alters bone development of their newborn offspring by hormonal modulation 10.1371/journal.pone.0179693
Journal of Controlled Release A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury pubmed:28522195
Obesity Surgery Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Mice pubmed:28853013
Journal of Nutritional Sciences Effect of dietary protein intake on the body composition and metabolic parameters of neutered dogs pubmed:29152244
Inflammation Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair. pubmed:28540539
Frontiers in Molecular Neuroscience Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis. pubmed:28959186
Journal of Photochemistry and Photobiology Various LED Wavelengths Affected Myofiber Development and Satellite Cell Proliferation of Chick Embryos via the IGF-1 Signaling Pathway. pubmed:28708285
Brain, Behavior, and Immunity An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling Pubmed:29627530
Journal of Translational Medicine Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer Pubmed:29482569
PLoS One Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588
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CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE 26S proteasome and insulin-like growth factor-1 in serum of dogs suffering from malignant tumors Pubmed:29755191
Pakistan Journal of Zoology Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. :
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Oncotarget Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy Pubmed:29423092
Science Citation Index Effect of porcine somatotropin on metabolism, testicular size and sperm characteristics in young boars 10.1590:1678-4162-9481
Journal of Animal Science Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs Pubmed: 30203098
Journal of Animal Physiology and Animal Nutrition Maternal HMB treatment affects bone and hyaline cartilage development in their weaned piglets via the leptin/osteoprotegerin system Pubmed: 30659706
Biomedicine & Pharmacotherapy The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKB Pubmed: 30784932
The American Journal of Tropical Medicine and Hygiene Association between Insulin-Like Growth Factor-I Levels and the Disease Progression and Anemia in Visceral Leishmaniasis Pubmed: 30761980
PLoS One Light-dark rhythms during incubation of broiler chicken embryos and their effects on embryonic and post hatch leg bone development Pubmed: 30682088
International Journal of Molecular Sciences Mel1c Mediated Monochromatic Light-Stimulated IGF-I Synthesis through the Intracellular Gαq/PKC/ERK Signaling Pathway Pubmed: 30987295
Animal Reproduction Science Serum and seminal plasma IGF-1 associations with semen variables and effect of IGF-1 supplementation on semen freezing capacity in buffalo bulls Pubmed: 30914211
PLoS One Melatonin mediates monochromatic green light-induced satellite cell proliferation and muscle growth in chick embryo Pubmed: 31059537
Journal of Innovations in Pharmaceutical and Biological Sciences Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and …
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Br J Nutr The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study 34348809
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Int J Environ Res Public Health Association of Salivary IGF and IGF/IGFBP-3 Molar Ratio with Cervical Vertebral Maturation Stages from Pre-Adolescent to Post-Adolescent Transition Period—A … Pubmed:35564566
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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EPA050Hu61 Eukaryotic Insulin Like Growth Factor 1 (IGF1) Positive Control; Immunogen; SDS-PAGE; WB.
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PAA050Hu01 Polyclonal Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC; IP.
PAA050Hu06 Polyclonal Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC; IP.
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MAA050Hu24 Monoclonal Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC; IP.
MAA050Hu26 Monoclonal Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC; IP.
MAA050Hu27 Monoclonal Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC; IP.
MAA050Hu28 Monoclonal Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC; IP.
RAA050Hu21 Recombinant Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC.
MAA050Hu22 Monoclonal Antibody to Insulin Like Growth Factor 1 (IGF1) WB,ICC/IF
RAA050Hu22 Recombinant Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC; FCM
MAA050Hu21 Monoclonal Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC; IP.
MAA050Hu25 Monoclonal Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC; IP.
MAA050Hu23 Monoclonal Antibody to Insulin Like Growth Factor 1 (IGF1) WB; IHC; ICC; IP.
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SEA050Hu ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA050Hu High Sensitive ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA050Hu CLIA Kit for Insulin Like Growth Factor 1 (IGF1) Chemiluminescent immunoassay for Antigen Detection.
LMA050Hu Multiplex Assay Kit for Insulin Like Growth Factor 1 (IGF1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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KSA050Hu01 ELISA Kit DIY Materials for Insulin Like Growth Factor 1 (IGF1) Main materials for "Do It (ELISA Kit) Yourself".