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Active Interleukin 12B (IL12B)

p40; IL12-B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit

ACTIVITY TEST

Interleukin 12B (IL12B)encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities.Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. Interleukin 12B can combind with Interleukin 12 Receptor Beta 1 (IL12Rb1). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL12B and recombinant human IL12Rb1. Briefly, biotin-linked recombinant human IL12B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl then transferred to IL12Rb1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with HRP conjugage for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of IL12B and IL12Rb1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Anticancer Research Establishment and evaluation of a bead-based luminex assay allowing simultaneous quantification of equine IL-12 and IFN-γ. Pubmed: 23564769
Scandinavian Journal of Immunology Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress. pubmed:27384769
Journal of Functional Foods Impact of purified oat 1-3, 1-4-β-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritis S1756464616303528
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Antibiotics Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal Pigs Pubmed: 31963653
Parasites & Vectors Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … Pubmed: 32680553
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA058Hu01 Active Interleukin 12B (IL12B) Cell culture; Activity Assays.
RPA058Hu01 Recombinant Interleukin 12B (IL12B) Positive Control; Immunogen; SDS-PAGE; WB.
PAA058Hu01 Polyclonal Antibody to Interleukin 12B (IL12B) WB; IHC; ICC; IP.
RAA058Hu21 Recombinant Antibody to Interleukin 12B (IL12B) WB; IF; ICC; IHC; IP; FCM.
MAA058Hu21 Monoclonal Antibody to Interleukin 12B (IL12B) WB; IHC; ICC; IP.
MAA058Hu22 Monoclonal Antibody to Interleukin 12B (IL12B) WB; IHC; ICC; IP.
MAA058Hu23 Monoclonal Antibody to Interleukin 12B (IL12B) WB; IHC; ICC; IP.
MAA058Hu24 Monoclonal Antibody to Interleukin 12B (IL12B) WB; IHC; ICC; IP.
MAA058Hu25 Monoclonal Antibody to Interleukin 12B (IL12B) WB; IHC; ICC; IP.
MAA058Hu27 Monoclonal Antibody to Interleukin 12B (IL12B) WB; IHC; ICC; IP.
SEA058Hu ELISA Kit for Interleukin 12B (IL12B) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA058Hu Multiplex Assay Kit for Interleukin 12B (IL12B) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.