≡

Active Interleukin 13 (IL13)

ALRH; BHR1; P600; IL-13; NC30

ACTIVITY TEST

Interleukin 13 (IL13) is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells. IL13 has effects on immune cells that are similar to those of the closely related cytokine IL4. Although IL13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. IL13 induces a class of protein-degrading enzymes, known as matrix metalloproteinases (MMPs), in the airways. Furthermore, IL-13 can induce immunoglobulin E (IgE) secretion from activated human B cells. Besides, Interleukin 13 Receptor Alpha 1 (IL13Ra1) has been identified as an interactor of IL13, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IL13 and recombinant mouse IL13Ra1. Briefly, biotin-linked IL13 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL13Ra1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of IL13 and IL13Ra1 was shown in Figure 1, the EC50 for this effect is 0.018 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Critical Care Medicine Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage PubMed: PMC3383056
Int J Clin Exp Pathol Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation PubMed: 26722390
Advances in Experimental Medicine and Biology  Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury Pubmed:27283193
Molecular Immunology Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-ŚĘB in murine model of asthma Pubmed:27289030
Journal of Agricultural Biotechnology Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells 10610
BMC Veterinary Research Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis pubmed:27553600
Molecular Immunology Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma pubmed:27289030
Physiological Research Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. pubmed:28006947
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847
Veterinary Immunology and Immunopathology Cytokine levels in colostrum and in foals' serum pre- and post-suckling pubmed:28242000
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice pubmed:28122397
Journal of Autoimmunity Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice 10.1016:j.jaut.2018.07.003
bulletin of experimental biology and medicine Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin Pubmed: 31183654
life sciences Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome Pubmed: 31738882
Frontiers in Immunology TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation Pubmed: 32117301
Parasites & Vectors Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism … Pubmed: 32680553
Biomedicine & Pharmacotherapy Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice Pubmed: 32521453
Journal of Traditional Chinese Medical Sciences Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice
Traditional Medicine Research Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
Applied materials today Tanshinone IIA-loaded aligned microfibers facilitate stem cell recruitment and capillary formation by inducing M2 macrophage polarization
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
Biomed Pharmacother Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice 33706133
BIOMATERIALS Aligned microfiber-induced macrophage polarization to guide schwann-cell-enabled peripheral nerve regeneration 33813259
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies 33999928
Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APA060Hu01 Active Interleukin 13 (IL13) Cell culture; Activity Assays.
EPA060Hu61 Eukaryotic Interleukin 13 (IL13) Positive Control; Immunogen; SDS-PAGE; WB.
RPA060Hu01 Recombinant Interleukin 13 (IL13) Positive Control; Immunogen; SDS-PAGE; WB.
PAA060Hu01 Polyclonal Antibody to Interleukin 13 (IL13) WB; IHC; ICC; IP.
RAA060Hu21 Recombinant Antibody to Interleukin 13 (IL13) WB; IF; ICC; IHC; IP; FCM.
MAA060Hu22 Monoclonal Antibody to Interleukin 13 (IL13) WB; IHC; ICC; IP.
SEA060Hu ELISA Kit for Interleukin 13 (IL13) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA060Hu Multiplex Assay Kit for Interleukin 13 (IL13) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA060Hu01 ELISA Kit DIY Materials for Interleukin 13 (IL13) Main materials for "Do It(ELISA Kit) Yourself"