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Active Superoxide Dismutase 3, Extracellular (SOD3)

SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn

ACTIVITY TEST

Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl)amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant rabbit SOD3. The reaction was performed in adding 8 μl 5 mmol/L pyrogallol to 200 μl 50mmol/L Tris-HCl, rapidly mixing at 25 ℃, then read at 325 nm (using 50mmol/L Tris-HCl as blank control) in kinetic mode for 3 minutes using a microplate reader controlling the pyrogallol autoxidation rate at 0.70 OD/min. Different concentrations of recombinant rabbit SOD3 were added into 200 μl 50 mmol/L Tris-HCl, incubated for 20 min at 25 ℃, then adding 8 μl 5 mmol/L pyrogallol to each well, rapidly mixing and read at 325 nm in kinetic mode for 3 minutes. Under these conditions, the enzyme amount of 50% inhibition of pyrogallol autooxidation per minute is defined as a unit. The specific activity of recombinant rabbit SOD3 is 133.9 U/mg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
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Nature Communications SOD3 improves the tumor response to chemotherapy by stabilizing endothelial HIF-2α Pubmed:29422508
BMC Neuroscience Neuroprotective dobutamine treatment upregulates superoxide dismutase 3, anti-oxidant and survival genes and attenuates genes mediating inflammation Pubmed:29523072
Diabetes-Metabolism Research and Reviews Myeloperoxidase, superoxide dismutase‐3, cardiometabolic risk factors, and distal sensorimotor polyneuropathy: The KORA F4/FF4 study Pubmed:29577557
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Clinical Kidney Journal Serum uromodulin is inversely associated with biomarkers of subclinical inflammation in the population-based KORA F4 study
PLoS One Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study Pubmed:34990470
ORIGINAL RESEARCH PATHOPHYSIOLOGY EFFECTS OF HIS-PHE-ARG-TRP-PRO-GLY-PRO PEPTIDE ON FREE-RADICAL OXIDATION PROCESSES IN CONDITIONS OF CHRONIC RESTRAINT …
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