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Active Stem Cell Factor (SCF)

KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2

ACTIVITY TEST

Figure. Cell proliferation of Raw264.7 cells after stimulated with SCF.
Stem cell factor (also known as SCF, KIT-ligand, KL, or steel factor) is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. SCF has been shown to  stimulate the proliferation of Raw264.7 cells. To test this effect, Raw264.7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach later, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM containing various concentrations of recombinant mouse SCF. After incubated for 120h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of Raw264.7 cells after incubation with SCF for 120h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant SCF for 120h. The result was shown in Figure 2. It was obvious that SCF significantly increased cell viability of Raw264.7 cells.
(A) Raw264.7 cells cultured in DMEM, stimulated with 1ng/mL SCF for 120h;
(B) Unstimulated Raw264.7 cells cultured in DMEM for 120h.

Figure. Cell proliferation of Raw264.7 cells after stimulated with SCF.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Archives of Gynecology and Obstetrics Effect of ovarian stimulation with recombinant follicle-stimulating hormone, gonadotropin-releasing hormone agonist and antagonists, on follicular fluid stem cell factor and serum urocortin 1 levels on the day of oocyte retrieval Pubmed: 23801011
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer PubMed: 26266370
Tumor Biology Hypoxia-induced secretion of IL-13 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151
Molecular Neurobiology Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology. pubmed:27730513
Oncotarget Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy Pubmed:29423092
Journal of Environmental Research and Public Health Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats Pubmed: 30974849
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA120Mu02 Recombinant Stem Cell Factor (SCF) Positive Control; Immunogen; SDS-PAGE; WB.
RPA120Mu01 Recombinant Stem Cell Factor (SCF) Positive Control; Immunogen; SDS-PAGE; WB.
APA120Mu01 Active Stem Cell Factor (SCF) Cell culture; Activity Assays.
PAA120Mu02 Polyclonal Antibody to Stem Cell Factor (SCF) WB; IHC; ICC; IP.
PAA120Mu01 Polyclonal Antibody to Stem Cell Factor (SCF) WB; IHC; ICC; IP.
LAA120Mu81 FITC-Linked Polyclonal Antibody to Stem Cell Factor (SCF) WB; IHC; ICC; IF.
LAA120Mu71 Biotin-Linked Polyclonal Antibody to Stem Cell Factor (SCF) WB; IHC; ICC.
MEA120Mu Mini Samples ELISA Kit for Stem Cell Factor (SCF) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA120Mu ELISA Kit for Stem Cell Factor (SCF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA120Mu CLIA Kit for Stem Cell Factor (SCF) Chemiluminescent immunoassay for Antigen Detection.
LMA120Mu Multiplex Assay Kit for Stem Cell Factor (SCF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.