Active Galactosidase Beta (GLb)
GL-B; b-GAL; bGAL; ELNR1; EBP; GLB1; Lactase; Elastin Receptor 1,67kDa; Elastin Receptor 1(67kD); Acid Beta-Galactosidase
- Product No.APA196Ca62
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point6.9
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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For more details, please contact local distributors!
ACTIVITY TEST
GLB1 is a lysosomal beta -galactosidase that hydrolyzes the terminal beta -galactose from ganglioside and keratan sulfate. Defects in this gene are the causes of lysosomal storage diseases for GM1-gangliosidosis and Morquio B syndrome (also known as mucopolysaccharidosis IVB). In GM1 gangliosidosis, GM1 ganglioside accumulates in the neurons of the central nervous system, because of the deficiency of lysosomal beta -galactosidase activity. GM1 gangliosidosis demonstrates varying degrees of clinical severity but is invariably fatal, and children with the most common and severe form of GM1 gangliosidosis usually die within 3 years of birth. Morquio B syndrome patients are neurologically normal, but display severe skeletal dysostosis multiplex because of an accumulation of keratan sulfate. The activity assay of GLB1 was measured by its ability to cleave a peptide substrate, 4-Methylumbelliferyl-beta -D-galactopyranoside. The reaction was performed in 50 mM Sodium Citrate, pH 3.5 ( Assay Buffer), ainitiated by addition 50 μL of 1.5 ug/ml GLB1 (diluted by Assay Buffer) to 50 µL of 1.2 mM substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant dog GLB1 is >9700 pmol/min/µg.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Buffer
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- Real Time PCR Experimental Service
- Spike RBD Protein (S-RBD)
- Protein G
- Protein A
Magazine | Citations |
DISSERTATIONEN ONLINE DER FREIEN UNIVERSITÄT BERLIN | Human epidermis reconstructed from UV-B irradiated keratinocytes mimics epidermal ageing FUDISS_derivate_000000020558 |
European Journal of Pharmaceutics and Biopharmaceutics | Increased permeability of reconstructed human epidermis from UVB-irradiated keratinocytes. pubmed:28034807 |
American Journal of Orthodontics and Dentofacial Orthopedics | Periodontal ligament cells in adolescents and adults: Genetic level responses to orthodontic forces Pubmed: 33082075 |
Eur J Orthod | Morphological alterations, activity, mRNA fold changes, and aging changes before and after orthodontic force application in young and adult human-derived ¡ 34041525 |
Catalog No. | Related products for research use of Canis familiaris; Canine (Dog) Organism species | Applications (RESEARCH USE ONLY!) |
APA196Ca62 | Active Galactosidase Beta (GLb) | Cell culture; Activity Assays. |
RPA196Ca01 | Recombinant Galactosidase Beta (GLb) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA196Ca01 | Polyclonal Antibody to Galactosidase Beta (GLb) | WB; IHC; ICC; IP. |