Packages (Simulation)
Image (I)
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Gene sequencing
Image (II)
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Figure. SDS-PAGE
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Figure. Western Blot; Sample: Recombinant AMH, Human.
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Instruction Manual
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Active Anti-Mullerian Hormone (AMH)
MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance
- Product No.APA228Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point7.7
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 346
US$ 864
US$ 1728
US$ 5184
US$ 12960
For more details, please contact local distributors!
ACTIVITY TEST
Anti-Müllerian hormone (AMH), also named Müllerian inhibiting substance (MIS) belongs to a tissue-specific TGF-beta superfamily growth factor. It can be expressed by male sertoli cells and postnatal testis, and ovarian granulosa cells of females postpartum. AMH expression is critical to sex differentiation at a specific time during fetal development, it appears to be tightly regulated by SF1, GATA factors, DAX1 and FSH. AMH signals through a characteristic receptor consisting of a type I and a type II receptor serine/threonine kinase. Especially the type II receptor is unique and specific receptor for AMH. Besides, Mothers Against Decapentaplegic Homolog 9 (Smad9) has been identified as an interactor of AMH, thus a binding ELISA assay was conducted to detect the interaction of recombinant human AMH and recombinant human (Smad9) Briefly, AMH were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Smad9-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AMH pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of AMH and Smad9 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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BCA Protein Quantification Kit
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Molecular Mass Marker for Protein
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
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Buffer
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Adenovirus Packaging Experiment Service
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
| Magazine | Citations |
| Journal of Zhejiang University SCIENCE B | Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice. PubMed: 23125082 |
| In Vivo.(Athens,Greece) | Association Between AMH, Oocyte Number and Availability of Embryos for Cryopreservation in IVF Pubmed: 24292596 |
| Gynecological Endocrinology | The effects of isotretinoin on the ovarian reserve of females with acne Informahealthcare: Source |
| Fertility and Sterility | Increased expression of anti mullerian hormone and its receptor in endometriosis Ingentaconnect: Source |
| Reproductive BioMedicine Online | Decreased ovarian reserve in female Sprague–Dawley rats induced by isotretinoin (retinoic acid) exposure Rbmojournal: Source |
| Reproductive Biology and Endocrinology | The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study. Pubmed: 24341292 |
| Biomed Res Int. | Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats Pubmed:Pmc4053297 |
| Fertil Steril. | Increased expression of antimüllerian hormone and its receptor in endometriosis Pubmed:24613539 |
| The Journal of Maternal-Fetal & Neonatal Medicine | Maternal serum anti-Müllerian hormone at 11-13 weeks' gestation in the prediction of preeclampsia Pubmed:24953352 |
| J Gynecol Oncol. | Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model Pubmed:25142624 |
| Reproductive BioMedicine Online | The effect of childhood cancer therapy on ovarian reserve and pubertal development Pubmed:25498597 |
| Reproductive Sciences | Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome Pubmed:25667201 |
| Yonsei Medical Journal | Anti-Proliferative and Apoptotic Activities of Müllerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines PubMed: 26632380 |
| oncology research | Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940 |
| Reprod Biomed Online | Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats PubMed: 26206282 |
| PLoS One | Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis PubMed: 26465611 |
| Toxicol Lett | Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood PubMed: 26657179 |
| Journal of Assisted Reproduction and Genetics | Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage? Pubmed:27255570 |
| Anticancer Res. | Prognostic Value of Anti-Müllerian Hormone and Inhibin B in Patients with Premenopausal Hormone Receptor-positive Breast Cancer Pubmed:26976997 |
| Chemosphere. | Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. pubmed:27567155 |
| Theriogenology | Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males. pubmed:28043339 |
| environmental pollution | Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061 |
| Journal of Assisted Reproduction and Genetics | Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. pubmed:28074436 |
| Journal Pakistan Medical Association | Serum anti-mullerian hormone: Correlation with the ovarian follicular dynamics in healthy mice pubmed:27654725 |
| Advance Medical Associates | Are anti-Müllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes? pubmed:27162065 |
| Yonsei Med J Volume | Anti-Proliferative and Apoptotic Activities of M? llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines. pubmed:26632380 |
| Journal of Endocrinological Investigation | The use of serum anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) to predictthe number of oocytes collected and availability of embryos for cryopreservation in IVF. pubmed:27465668 |
| Journal of the Pakistan Medical Association | Predictive accuracy of anti mullerian hormone as indicator of ovarian follicle loss in cyclophosphamide treated mice pubmed:28955058 |
| International Journal of Reproduction, Contraception, Obstetrics and Gynecology | Can Co-enzyme Q10 improve the chances of conception after the age of 35? 10.18203/2320-1770.ijrcog20172902 |
| Animal Production Science | Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? 10.1071/ANv57n12Ab065 |
| Reproduction. | Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593 |
| Theriogenology | Relationship among circulating anti-Müllerian hormone, insulin like growth factor 1, cadmium and superovulatory response in dairy cows. pubmed:28708536 |
| Journal of ovarian research | Rapamycin Prevents cyclophosphamide-induced Over-activation of Primordial Follicle pool through PI3K/Akt/mTOR Signaling Pathway in vivo. pubmed:28814333 |
| Annals of Rheumatic Diseases | FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study Suppl_2:728.1.abstract |
| Free Radical Biology & Medicine | Peroxiredoxin 2 deficiency accelerates age-related ovarian failure through the reactive oxygen species-mediated JNK pathway in mice Pubmed:29778464 |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625 |
| Biology of Reproduction | Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss Pubmed:29351580 |
| Endocrine Connections | Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery Pubmed:30012648 |
| Reproductive BioMedicine Online | Dienogest suppresses the activation of primordial follicles and preserves the primordial follicle stockpile for fertility in mice Pubmed:29609766 |
| Journal of Ovarian Research | Comparison of the influence of three fibroid treatment options: supracervical hysterectomy, ulipristal acetate and uterine artery embolization on ovarian … Pubmed:29859107 |
| Animal Reproduction Science | Anti-Müllerian hormone and Oestradiol as markers of future reproductive success in juvenile gilts Pubmed:29859701 |
| Stem Cell Research & Therapy | Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243 |
| Biochemical and Biophysical Research Communications | Involvement of obesity-associated upregulation of chemerin/chemokine-like receptor 1 in oxidative stress and apoptosis in ovaries and granulosa cells Pubmed: 30722991 |
| Lupus | Levels of anti-Müllerian hormone in premenopausal women with the antiphospholipid syndrome and its association with the risk of clinical complications Pubmed: 30717622 |
| In Vitro Cellular & Developmental Biology - Animal | Transplantation of umbilical cord–derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice Pubmed: 30820812 |
| Biomedicine & Pharmacotherapy | Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve |
| Reproductive Biology and Endocrinology | Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … Pubmed: 31311554 |
| Oxidative Medicine and Cellular Longevity | Carvacrol and Thymol Modulate the Cross-Talk between TNF-α and IGF-1 Signaling in Radiotherapy-Induced Ovarian Failure |
| Acta Obstet Gynecol Scand | The impact of uterine artery embolization on ovarian reserve: a systematic review and meta‐analysis Pubmed: 31370100 |
| Reproductive Sciences | Uterine Cells Improved Ovarian Function in a Murine Model of Ovarian Insufficiency Pubmed: 31530098 |
| Animals | Serum Concentrations of AMH and E2 and Ovarian and Uterine Traits in Gilts Pubmed: 31619004 |
| Journal Of Pakistan Medical Association | The effect of acupuncture on anti-mullerian hormone and assisted reproduction outcome in Polycystic Ovary Syndrome patients undergoing in vitro fertilization … Pubmed: 31603867 |
| In Vitro Cellular & Developmental Biology | Basal characterization and in vitro differentiation of putative stem cells derived from the adult mouse ovary Pubmed: 31900800 |
| J Assist Reprod Genet | The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage Pubmed: 32020412 |
| Biology (Basel). | Seminal Plasma Anti-Müllerian Hormone: A Potential AI-Boar Fertility Biomarker? Pubmed: 32290279 |
| Eur J Breast Health | The Effect of Systemic Chemotherapy on Ovarian Function: A Prospective Clinical Trial Pubmed: 32656517 |
| Heliyon | Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting … Pubmed: 33005806 |
| CYTOMETRY PART A | After cyclophosphamide exposure, granulosa cells recover their anti©\M¨¹llerian hormone©\producing ability but not their numbers 33342073 |
| BIOLOGY OF REPRODUCTION | Igf3 is essential for ovary differentiation in zebrafish 33276384 |
| JOURNAL OF ETHNOPHARMACOLOGY | Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis 33422655 |
| Stem Cells Int | Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … 34733331 |
| Sci Total Environ | Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression Pubmed:35276145 |
| Hum Reprod Update | Anti-Müllerian hormone as a marker of ovarian reserve and premature ovarian insufficiency in children and women with cancer: a systematic review Pubmed:35199161 |
| Horm Metab Res | Anti-Mullerian Hormone Levels Increase After Bariatric Surgery in Obese Female Patients With and Without Polycystic Ovary Syndrome Pubmed:35276745 |
| American Journal of Translational Research | The effects of vasopressin injection technique on ovarian reserve in laparoscopic cystectomy of bilateral ovarian endometrioma: a retrospective cohort study Pubmed:35559399 |
| Stem Cell Research & Therapy | G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and … |
| Research Square | Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency |
| Fundamental Research | Long-term amelioration of an early-onset familial atrial fibrillation model with AAV-mediated in vivo gene therapy |