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Active Trypsin (TRY)

ACTIVITY TEST

Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyzes proteins. Trypsin cuts peptide chains mainly at the carboxyl side of the amino acids lysine or arginine. It is used for numerous biotechnological processes. The connection between animal cells is a specific connection formed by the differentiation of the plasma membrane (cell membrane) between adjacent cells. The plasma membrane is mostly composed of protein-lipid-protein unit membrane, and the proteins on the plasma membrane are mostly involved in the connection. Thus, the digestion of adherent cells HepG2 was to detect the bioactivity of recombint pig trypsin. HepG2 cells were seeded into 96-well plates at a density of 4,000 cells/well with 10% serum standard DMEM overnight. Various concentrations of recombinant pig trypsin was added into the HepG2 after washed by PBS buffer. Cells were observed by inverted microscope after a period of time cultured at 37℃. The result was shown in Figure 1. It was obvious that recombinant pig trypsin can digest the HepG2, the minimum protein concentration was 46.9 ug/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
NPA250Po01 Native Trypsin (TRY) Positive Control; Immunogen; SDS-PAGE; WB.
RPA250Po01 Recombinant Trypsin (TRY) Positive Control; Immunogen; SDS-PAGE; WB.
APA250Po51 Active Trypsin (TRY) Cell culture; Activity Assays.
PAA250Po02 Polyclonal Antibody to Trypsin (TRY) WB; IHC; ICC; IP.
PAA250Po01 Polyclonal Antibody to Trypsin (TRY) WB
LAA250Po71 Biotin-Linked Polyclonal Antibody to Trypsin (TRY) WB; IHC; ICC.
MAA250Po21 Monoclonal Antibody to Trypsin (TRY) WB; IHC; ICC; IP.
MAA250Po23 Monoclonal Antibody to Trypsin (TRY) WB; IHC; ICC; IP.
MAA250Po24 Monoclonal Antibody to Trypsin (TRY) WB; IHC; ICC; IP.
MAA250Po22 Monoclonal Antibody to Trypsin (TRY) WB; IHC; ICC; IP.
SEA250Po ELISA Kit for Trypsin (TRY) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA250Po CLIA Kit for Trypsin (TRY) Chemiluminescent immunoassay for Antigen Detection.
LMA250Po Multiplex Assay Kit for Trypsin (TRY) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA250Po01 Antibody Pair for Trypsin (TRY) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA250Po01 ELISA Kit DIY Materials for Trypsin (TRY) Main materials for "Do It (ELISA Kit) Yourself".