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Active Galectin 2 (GAL2)

LGALS2; HL14; Lectin,Galactoside-Binding Soluble 2; Beta-galactoside-binding lectin L-14-II; Lactose-binding lectin 2; S-Lac lectin 2

ACTIVITY TEST

Galectin 2 (GAL2) belongs to the proto type group and consists of two homologous carbohydrate recognition domains (CRDs) resulting in multiple sugar binding sites. The expression of gal-2 has been shown to be involved in processes of angiogenesis and inflammation but was not analyzed before in preeclamptic (PE) placentas.It also can agglutinate red blood. In this case, chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant rat GAL2 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL2 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL2, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL2 is 1.25μg/ml.

Figure 2. The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Biol Pharm Bull Potential Interaction between Galectin-2 and MUC5AC in Mouse Gastric Mucus Pubmed: 32009121
International Journal of Molecular Sciences Galectin-2 Has Bactericidal Effects against Helicobacter pylori in a β-galactoside-Dependent Manner Pubmed: 32295066
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APA302Ra01 Active Galectin 2 (GAL2) Cell culture; Activity Assays.
RPA302Ra01 Recombinant Galectin 2 (GAL2) Positive Control; Immunogen; SDS-PAGE; WB.
PAA302Ra01 Polyclonal Antibody to Galectin 2 (GAL2) WB; IHC; ICC; IP.