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Active Galectin 1 (GAL1)

LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin

ACTIVITY TEST

Galectins are a family of carbohydrate-binding proteins with an affinity for beta-galactosides. Galectin-1 (GAL1) is differentially expressed by various normal and pathological tissues and appears to be functionally polyvalent, with a wide range of biological activity. The intracellular and extracellular activity of GAL1 has been described. Evidence points to GAL1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.It also can agglutinate red blood. In this case, chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL1 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL1 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL1, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL1 is 2.5μg/mL.

Figure 2. The hemagglutination assay of GAL1 in V- bottom shaped 96-well microtiter plate.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
International Journal of Oncology Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: Regulatory roles of cell surface glycans Pubmed:24589677
Clinica Chimica Acta Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population Pubmed:25578395
Journal of Perinatal Medicine Cord blood galectin-1 and-3 concentrations in term pregnancies with normal restricted and increased fetal growth PubMed: 24717333
Biomaterials Noninvasive small-animal imaging of galectin-1 upregulation for predicting tumor resistance to radiotherapy Pubmed:29272764
Advances in Experimental Medicine and Biology Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease Pubmed:29429028
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY Elevated level of Galectin-1 in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis Pubmed: 31678459
Eur J Nucl Med Mol Imaging Galectin expression detected by 68Ga-galectracer PET as a predictive biomarker of radiotherapy resistance Pubmed:35106644
Medicine Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … Pubmed:35687771
In Vitro Bovine Embryo Production Using Medium's Supplemented with Recombinant Human Galectin-1 Buffered
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